在这项工作中,已经评估了γ辐照的表面活性剂对药物-蛋白质结合的影响。使用PluronicF-127、PluronicL-35、吐温20和吐温80表面活性剂的辐照水溶液。进行了三种不同剂量的伽马射线照射,这四种表面活性剂即。,6、30和36kGy。两种药物,奥硝唑(ONZ)和替米沙坦(TMS)用于结合研究。分析了在药物-牛血清白蛋白(BSA)蛋白存在下四种辐照表面活性剂的作用。在初始步骤中将在甲醇-水性介质中的药物溶液与BSA合并。在接下来的两个步骤中,在未辐照和辐照表面活性剂存在下进行药物-BSA相互作用。比较了由于添加了辐照和未辐照表面活性剂而导致的药物-BSA的结果。通过紫外光谱法评估相互作用过程,DLS,zeta电位,浊度,和对接研究。如使用UV光谱研究从结合常数值确定的,对于经辐照的表面活性剂,观察到药物和四种表面活性剂的改善的结合。DLS测量表明,随着吸收剂量,胶束尺寸没有增加或减少的一般趋势。观察到胶束的结合和大小变化是高度药物和表面活性剂特异性的。在四种表面活性剂中,辐照的PluronicF-127显示出更高的结合亲和力。据我们所知,这是首次报道γ辐照表面活性剂对药物-BSA相互作用的影响。这可以应用于其他药物-蛋白质系统以将它们的相互作用调节到所需水平。由RamaswamyH.Sarma沟通。
In this work, the effect of γ-irradiated surfactants on drug-protein binding has been assessed. Irradiated aqueous solutions of Pluronic F-127, Pluronic L-35, Tween 20, and Tween 80 surfactants were used. Gamma irradiation was carried out for three different doses, to these four surfactants viz., 6, 30, and 36 kGy. Two drugs, Ornidazole (ONZ) and Telmisartan (TMS) were used for the binding study. The effect of four irradiated surfactants in the presence of drug - Bovine serum albumin (BSA) protein was analyzed. The drug solutions in methanol-aqueous media were combined with BSA in the initial step. In the next two succeeding steps, drug-BSA interaction in the presence of unirradiated and irradiated surfactants were carried out. The results of drug-BSA due to addition of irradiated and unirradiated surfactants were compared. The interaction processes were assessed through UV Spectroscopy, DLS, zeta potential, turbidity, and docking studies. Improved binding was observed for both the drugs and four surfactants for irradiated surfactants as determined from the binding constant values using UV spectroscopic studies. The DLS measurements demonstrated no general trend of increase or decrease in micellar size with absorbed dose. The binding and change in the size of micelles were observed to be highly drug and surfactant-specific. Among the four surfactants, irradiated Pluronic F-127 showed higher binding affinity. To the best of our knowledge, this is the first report on the effect of γ-irradiated surfactant on drug-BSA interaction. This can be applied to other drug-protein systems to tune their interaction to the required level.Communicated by Ramaswamy H. Sarma.