目的:乌贼弯曲菌已被认为是一种新兴的病原菌。然而,人们对它在环境中的生存知之甚少。为了评估它的生存能力,我们估计在磷酸盐缓冲盐水(PBS)中有氧暴露于饥饿后,酸度(pH=4.3),高渗透压(4%NaCl),和干燥在不同温度下的湿浆盘。此外,评估了不同温度下狗粪便和狗粮的存活率。
结果:在25°C的4天和4°C的10周的饥饿条件下,乌贼弯曲杆菌仍可培养。在暴露于高渗透压9天后,干燥5天,和酸度2天,分别。同样,在25°C下在狗的粪便和狗的食物中存活了几天,在4°C下存活了几周。生物体的生存能力取决于含水量,还有温度。值得注意的是,与用作对照的空肠杆菌菌株相比,在所有测试条件下,测试的普氏杆菌菌株的弹性较小。研究结果表明,乌贼能够在各种环境胁迫下生存,这表明它可能对公众健康构成潜在威胁。
OBJECTIVE: Campylobacter upsaliensis has been recognized as an emerging pathogen. However, little is known about its survival in the environment. To evaluate its survival capability, we estimated the reduction in viable counts of C. upsaliensis after aerobic exposure to starvation in phosphate-buffered saline (PBS), acidity (pH = 4.3), high osmolarity (4% NaCl), and dryness in wet pulp disks at different temperatures. Also, survival in dog feces and dog food at variable temperate was assessed.
RESULTS: Campylobacter upsaliensis remained culturable under starvation for 4 days at 25 °C and for 10 weeks at 4 °C. C. upsaliensis was also recoverable after exposure to high osmolality for 9 days, dryness for 5 days, and acidity for 2 days, respectively. Similarly, C. upsaliensis survived in dog feces and dog food for several days at 25 °C and weeks at 4 °C. The survival capability of the organism was dependent on the water content, and also temperature. Notably, the tested C. upsaliensis strain was less resilient under all tested conditions than a C. jejuni strain used as a control. The findings showed that C. upsaliensis is able to survive under various environmental stresses, suggesting that it could pose a potential threat to public health.