■纳杰夫省被记录为癌症增加的伊拉克污染最严重的省份之一,自身免疫,堕胎案件。研究小组。根据纳杰夫省不同地理区域的居民情况,对来自三个测试组的88名成年志愿者进行了划分。第1组(G1;n,29)Al-Ansar的居民,Al-Abbaseyh,和Al-Manathera区,第2组(G2;n,27)该省22个不同分散地区的居民,第3组(G3;n,32)老纳杰夫市库法市和中心区的居民。根据以前的作者的发现,所有参与者的尿液和血液样本中都有铀污染,而且,根据尿8-OHdG化合物的水平,他们有DNA损伤。对照组4(G4;n,25)是苏莱曼尼亚省居民的成年健康伊拉克志愿者,铀污染程度低。本研究旨在通过评估血清白细胞介素(IL)-6,干扰素-γ(IFN-γ)的水平来确定铀污染和DNA损伤对免疫系统功能的影响。和IL-1β(β)。
■酶联免疫吸附测定(ELISA)(夹心法技术)用于评估测试组和对照组的血清细胞因子水平。
■据报道,与对照组相比,细胞因子水平显着升高(p≤0.01)。IL-6水平为764.64±24.12pg/ml,768.87±19.64pg/ml,G1、G2和G3分别为735.62±18.47。IFN-γ水平为264.55±19.17pg/ml,259±18.76pg/ml,G1、G2和G3分别为261.20±12.99pg/ml。IL-1β水平为99.85±10.81pg/ml,116.8±10.71pg/ml,G1、G2和G3分别为83±19.24pg/ml。IL-6、IFN-γ、IL-1β为86.5±22.9pg/ml,19.4±2.8pg/ml,和对照血清中的16.1±3.2pg/ml(G4)。结果显示IL-6、IFN-γ与相应的p值截止p≤0.01有显著的统计学升高,3个试验组血清中IL-1β和IL-1β水平与对照组比较。
■促炎细胞因子(IL-6,IFN-γ,和IL-1β)水平表明参与者持续的炎症反应,并可能反映长期低剂量电离辐射暴露导致的免疫系统受损。
UNASSIGNED: Najaf governorate was recorded as one of the most polluted Iraqi governorates with increased cancer, autoimmune, and abortion cases. Study Groups. A total of 88 adult volunteers from three test groups were divided based on their inhabitance in different geographical regions in Najaf governorate. Group 1 (G1; n, 29) inhabitants of Al-Ansar, Al-Abbaseyeh, and Al-Manathera districts, Group 2 (G2; n, 27) inhabitants of 22 different scattered districts of the governorate, Group 3 (G3; n, 32) inhabitants of Kufa city and center districts in the old Najaf city. According to previous authors\' findings, all participants had uranium contamination in their urine and blood samples, and also, they had DNA damage according to the level of urinary 8-OHdG compound. The control group 4 (G4; n, 25) were adult healthy Iraqi volunteers who were residents of the Sulaimaniyah governorate, which has low-level uranium pollution. The present study aims to determine the effect of uranium pollution and DNA damage on the immune system function in terms of estimating the levels of serum interleukin (IL)-6, interferon-gamma (IFN-γ), and IL-1 beta (β).
UNASSIGNED: Enzyme-linked immunosorbent assay (ELISA) (Sandwich method technique) was used for estimating the serum cytokines levels in test and control groups.
UNASSIGNED: A significant elevation of cytokines levels was reported as compared with the control groups (p ≤ 0.01). The level of IL-6 was 764.64 ± 24.12 pg/ml, 768.87 ± 19.64 pg/ml, and 735.62 ± 18.47 in G1, G2, and G3, respectively. The level of IFN-γ was 264.55 ± 19.17 pg/ml, 259 ± 18.76 pg/ml, and 261.20 ± 12.99 pg/ml for G1, G2, and G3, respectively. The level of IL-1β was 99.85 ± 10.81 pg/ml, 116.8 ± 10.71 pg/ml, and 83 ± 19.24 pg/ml in G1, G2, and G3, respectively. The levels of IL-6, IFN-γ, and IL-1β were 86.5 ± 22.9 pg/ml, 19.4 ± 2.8 pg/ml, and 16.1 ± 3.2 pg/ml in the sera of control (G4). The results showed significant statistical elevation with the corresponding p value cut-off p ≤ 0.01 in IL-6, IFN-γ, and IL-1β in the sera of three test groups as compared with the results of the control group.
UNASSIGNED: The change in the proinflammatory cytokines (IL-6, IFN-γ, and IL-1β) levels indicates a persistent inflammatory response in the participants and may reflect immune system impairment as a consequence of exposure to long-term low-dose ionizing radiation.