目的:烟酰胺磷酸核糖基转移酶(Nampt)是一种在肥胖中升高的脂肪细胞因子,2型糖尿病和水平升高与炎症过程有关。有人建议Nampt血清浓度在瘦男性的下午达到昼夜节律。然而,没有关于性别和体重影响的数据.
方法:我们测量了一组体重正常或肥胖的健康个体在24小时内的Nampt血清水平。此外,膳食的影响,口服葡萄糖耐量试验和体育锻炼对Nampt浓度进行了评价。进行了与其他激素和实验室参数以及人体测量的相关分析。
结果:Nampt表现出昼夜节律,白天水平升高,下午早些时候达到高峰。除肥胖男性外,这种昼夜节律对所有组都很重要。Nampt振幅,相对和绝对测量,女性明显高于男性。膳食不影响Nampt血清水平,而体育锻炼和OGTT确实显着影响Nampt血清水平。
结论:结论:我们发现Nampt振幅和系数变异的性别差异在女性中都较高。在健康的瘦个体中,Nampt的昼夜节律与性别无关,而肥胖男性则受到干扰。
OBJECTIVE: Nicotinamide phosphoribosyltransferase (Nampt) is an adipocytokine that is elevated in obesity, type 2 diabetes and increased levels are associated with inflammatory processes. Nampt serum concentrations have been suggested to follow a diurnal rhythm peaking in the afternoon in lean males. However, no data exists regarding the effects of gender and body weight.
METHODS: We measured Nampt serum levels over 24 h in a cohort of healthy individuals living with either normal weight or obesity. Furthermore, effects of meals, oral glucose tolerance test and physical exercise on Nampt concentrations were evaluated. Correlation analyses to other hormonal- and lab parameters and anthropometric measurements were performed.
RESULTS: Nampt showed a diurnal rhythm with increased levels at daytime and a peak in the early afternoon. This diurnal rhythm was significant for all groups but obese males. The Nampt amplitude, measured both relatively and absolutely, was significantly higher in females than in males. Meals did not influence Nampt serum levels, whereas physical exercise and an OGTT did significantly influence Nampt serum levels.
CONCLUSIONS: In conclusion, we found gender specific differences in Nampt amplitude and coefficient variation with both being higher in females. The circadian rhythm of Nampt was independent of gender in healthy lean individuals, whereas it was disturbed in men with obesity.