未经批准:系统发育基因组学已被广泛用于解决植物物种和属之间模棱两可且有争议的进化关系,以及在质体中识别独特的indel甚至可能有助于理解一些植物家族的进化。MenispermumL.(Menispermaceae)由三个物种组成,M.dauricumDC.,M.加拿大L.,还有墨西哥M.Rose,它们在东亚之间分离分布,北美东部和墨西哥。分类学家继续争论墨西哥分枝杆菌是否是一个独特的物种,各种各样的M.dauricum,或者仅仅是M.Canadense的同义词.迄今为止,没有分子系统学研究将这种可疑物种纳入系统发育分析。
未经批准:在这项研究中,我们使用29个半潜科的整个质体和18个半潜科的ITS1和ITS2序列,研究了整个范围内半潜科的系统基因组学和系统地理学。我们重建了Menispermum的种间关系,并探索了Menispermaceae的质体进化,揭示了该家族的几个基因组热点区域。
UNASSIGNED:基于整个质体和ITS1和ITS2序列的系统进化和网络分析表明,Menispermum聚集成两个支持值很高的进化枝,进化枝A(M.dauricum)andCladeB(M.加拿大+墨西哥M.)。然而,墨西哥分枝杆菌嵌套在加拿大分枝杆菌中,因此,我们支持墨西哥分枝杆菌是加拿大分枝杆菌的同义词。我们还确定了半春科的质体中的重要分子变异。在Menispermaceae中发生了几个indel,因此基因的过早终止。总共有54个区域被确定为最高变异的质体区域,核苷酸多样性(Pi)值>0.05,包括两个编码基因(matK,ycf1),四个内含子(trnK内含子,rpl16内含子,rps16内含子,ndhA内含子),和48个基因间间隔区(IGS)区。其中,四个信息丰富的热点区域(trnH-psbA,ndhF-rpl32,trnK-rps16和trnP-psaJ)对于未来的系统发育研究尤其有用,根茎科的系统地理学和保护遗传学。
UNASSIGNED: Phylogenomics have been widely used to resolve ambiguous and controversial evolutionary relationships among plant species and genera, and the identification of unique indels in plastomes may even help to understand the evolution of some plant families. Menispermum L. (Menispermaceae) consists of three species, M. dauricum DC., M. canadense L., and M. mexicanum Rose, which are disjuncly distributed among East Asia, Eastern North America and Mexico. Taxonomists continue to debate whether M. mexicanum is a distinct species, a variety of M. dauricum, or simply a synonym of M. canadense. To date, no molecular systematics studies have included this doubtful species in phylogenetic analyses.
UNASSIGNED: In this study, we examined phylogenomics and phylogeography of Menispermum across its entire range using 29 whole plastomes of Menispermaceae and 18 ITS1&ITS2 sequences of Menispermeae. We reconstructed interspecific relationships of Menispermum and explored plastome evolution in Menispermaceae, revealing several genomic hotspot regions for the family.
UNASSIGNED: Phylogenetic and network analyses based on whole plastome and ITS1&ITS2 sequences show that Menispermum clusters into two clades with high support values, Clade A (M. dauricum) and Clade B (M. canadense + M. mexicanum). However, M. mexicanum is nested within M. canadense and, as a result, we support that M. mexicanum is a synonym of M. canadense. We also identified important molecular variations in the plastomes of Menispermaceae. Several indels and consequently premature terminations of genes occur in Menispermaceae. A total of 54 regions were identified as the most highly variable plastome regions, with nucleotide diversity (Pi) values > 0.05, including two coding genes (matK, ycf1), four introns (trnK intron, rpl16 intron, rps16 intron, ndhA intron), and 48 intergenic spacer (IGS) regions. Of these, four informative hotspot regions (trnH-psbA, ndhF-rpl32, trnK-rps16, and trnP-psaJ) should be especially useful for future studies of phylogeny, phylogeography and conservation genetics of Menispermaceae.