(1)背景:本研究的目的是确定阿尔及利亚医院环境中梭状芽胞杆菌菌株的患病率,并评估其对抗生素和杀生物剂的抗菌敏感性。(2)方法:从特莱姆森大学医院的重症监护病房和外科病房的表面收集了500个表面样本,阿尔及利亚。使用MALDI-TOF-MS进行细菌鉴定,然后通过E-test方法确定各种抗菌剂的最低抑菌浓度(MIC)。通过酶促和PCR测定搜索sordellii毒素。对医院中用于日常消毒的七种产品进行了梭菌属的测试。孢子集合。(3)结果:在100个分离物中,鉴定出90例。所有菌株都缺乏致死和出血性毒素基因。β-内酰胺,利奈唑胺,万古霉素,替加环素,利福平,和氯霉素都证明对分离的菌株有效。在所有测试的菌株中,与其他梭状芽孢杆菌物种相比,索氏芽孢杆菌的孢子对测试的杀生物剂表现出明显的抗性。(氯基0.6%,30分钟),(戊二醛溶液2.5%,30分钟),和(过氧化氢/过氧乙酸3%,15分钟)的产品实现了所需的孢子减少。(4)结论:我们医院目前的清洁和消毒方法需要进行优化,以有效地清除照顾者手上的孢子,设备,和表面。
(1) Background: The purpose of this study was to determine the prevalence of clostridia strains in a hospital environment in Algeria and to evaluate their antimicrobial susceptibility to antibiotics and biocides. (2) Methods: Five hundred surface samples were collected from surfaces in the intensive care unit and surgical wards in the University Hospital of Tlemcen, Algeria. Bacterial identification was carried out using MALDI-TOF-MS, and then the minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by the E-test method. P. sordellii toxins were searched by enzymatic and PCR assays. Seven products intended for daily disinfection in the hospitals were tested against Clostridium spp. spore collections. (3) Results: Among 100 isolates, 90 P. sordellii were identified, and all strains were devoid of lethal and hemorrhagic toxin genes. Beta-lactam, linezolid, vancomycin, tigecycline, rifampicin, and chloramphenicol all proved effective against isolated strains. Among all strains tested, the spores of P. sordellii exhibited remarkable resistance to the tested biocides compared to other Clostridium species. The (chlorine-based 0.6%, 30 min), (glutaraldehyde solution 2.5%, 30 min), and (hydrogen peroxide/peracetic acid 3%, 15 min) products achieved the required reduction in spores. (4) Conclusions: Our hospital\'s current cleaning and disinfection methods need to be optimized to effectively remove spores from caregivers\' hands, equipment, and surfaces.