TilletiaindicaMitra通过致病性双核相在小麦中引起Karnalbunt(KB)。本研究是第一个使用Illumina和PacBio读数提供双核子(PSWKBGD-3)及其两个单孢系(PSWKBGH-1和2)基因组草案的研究,通过提取多态性SSR标记,对它们进行注释和三个基因组之间的比较分析。在24小时时,来自易感小麦品种WL711的受感染小麦籽粒的转录组,48h,接种PSWKBGH-1、2和PSWKBGD-3后7d也被分离。Further,利用T.indica转录组进行了两个转录组分析,以提取负责发病的二核基因,和小麦转录组,以提取受小麦KB进展过程中植物与病原体相互作用的二核影响的小麦基因。在24hai共有54、529和87个基因,48hai,和7dai,分别在二核阶段上调,而在24hai,48hai,和7dai,分别,仅在核子阶段被激活。同时,24hai共有23、17和52个小麦基因,48hai,和7dai,分别由于仅存在二核阶段而上调。在这项研究中获得的结果已在名为TiGeR(http://backlin。cabgrid.res.在/老虎/),这是T.indica分类基因的第一个基因组资源,三个T.in系的基因组和多态性SSR,在不同时间点KB的发生过程中,小麦和T.indicaDEGs以及受T.in.dikaryon的致病相关蛋白影响的小麦基因。本研究将有助于了解在KB的发展过程中,双核子在植物-病原体相互作用中的作用。这将有助于管理小麦中的KB,并开发抗KB小麦品种。
Tilletia indica Mitra causes Karnal bunt (KB) in wheat by pathogenic dikaryophase. The present study is the first to provide the draft genomes of the
dikaryon (PSWKBGD-3) and its two monosporidial lines (PSWKBGH-1 and 2) using Illumina and PacBio reads, their annotation and the comparative analyses among the three genomes by extracting polymorphic SSR markers. The trancriptome from infected wheat grains of the susceptible wheat cultivar WL711 at 24 h, 48h, and 7d after inoculation of PSWKBGH-1, 2 and PSWKBGD-3 were also isolated. Further, two transcriptome analyses were performed utilizing T. indica transcriptome to extract
dikaryon genes responsible for pathogenesis, and wheat transcriptome to extract wheat genes affected by
dikaryon involved in plant-pathogen interaction during progression of KB in wheat. A total of 54, 529, and 87 genes at 24hai, 48hai, and 7dai, respectively were upregulated in
dikaryon stage while 21, 35, and 134 genes of T. indica at 24hai, 48hai, and 7dai, respectively, were activated only in dikaryon stage. While, a total of 23, 17, and 52 wheat genes at 24hai, 48hai, and 7dai, respectively were upregulated due to the presence of dikaryon stage only. The results obtained during this study have been compiled in a web resource called TiGeR ( http://backlin.cabgrid.res.in/tiger/ ), which is the first genomic resource for T. indica cataloguing genes, genomic and polymorphic SSRs of the three T. indica lines, wheat and T. indica DEGs as well as wheat genes affected by T. indica dikaryon along with the pathogenecity related proteins of T. indica
dikaryon during incidence of KB at different time points. The present study would be helpful to understand the role of dikaryon in plant-pathogen interaction during progression of KB, which would be helpful to manage KB in wheat, and to develop KB-resistant wheat varieties.