培养肉是一种通过细胞培养而不是动物饲养来制造肉的新兴技术。在大多数现有的文化体系下,体外产生的肌管的含量和成熟度不足,限制养殖肉的应用和公众接受。在这里,我们证明了一种天然化合物,柚皮苷(NAR),促进了猪卫星细胞(PSC)体外成肌分化,增加了生成肌管的含量和成熟度,特别是对于经历了广泛扩增的PSC。机械上,NAR通过激活雌激素受体β在PSC的肌生成过程中上调IGF-1/AKT/mTOR合成代谢途径。此外,将PSC与水凝胶混合并在具有平行微通道的模具中培养以制造培养的猪肉样品。检测到更成熟的肌球蛋白,当分化培养基补充NAR时,观察到明显的肌节。一起来看,这些发现表明,NAR通过上调IGF-1信号诱导PSC的分化和成熟肌管的生成,促进高效和创新的养殖肉类生产系统的发展。
Cultured meat is an emerging technology for manufacturing meat through cell culture rather than animal rearing. Under most existing culture systems, the content and maturity of in vitro generated myotubes are insufficient, limiting the application and public acceptance of cultured meat. Here we demonstrated that a natural compound, naringenin (NAR), promoted myogenic differentiation of porcine satellite cells (PSCs) in vitro and increased the content and maturity of generated myotubes, especially for PSCs that had undergone extensive expansion. Mechanistically, NAR upregulated the IGF-1/AKT/mTOR anabolic pathway during the myogenesis of PSCs by activating the estrogen receptor β. Moreover, PSCs were mixed with hydrogels and cultured in a mold with parallel micro-channels to manufacture cultured pork samples. More mature myosin was detected, and obvious sarcomere was observed when the differentiation medium was supplemented with NAR. Taken together, these findings suggested that NAR induced the differentiation of PSCs and generation of mature myotubes through upregulation of the IGF-1 signaling, contributing to the development of efficient and innovative cultured meat production systems.