■组织工程是尝试通过不同方法恢复受损组织的应用系统,比如细胞治疗,细胞差异因子的应用,和各种材料。组织工程的重要目标之一是将干细胞直接引导到所需的组织,研究人员试图利用不同的分子作为改进这项技术的有效因素。
■本研究旨在证明双醋瑞因的作用,一种治疗骨关节炎的缓慢作用的药物,间充质干细胞增殖并评估其在软骨形成过程中的潜力。
■从脂肪组织中分离干细胞,以流式细胞术为特征,和细胞用10-5M双醋瑞因处理三周。SOX9、COL2A1、ACAN、通过qRT-PCR和免疫细胞化学技术分析TGFB1。
■我们的结果表明双醋瑞因增加了与软骨形成有关的以下基因的表达:SOX9(2.9倍,P<0.00),COL2A1(2.2倍,P<0.00),ACAN(2.7倍,P<0.00),和TGFB1(2.6倍,P<0.00)。免疫细胞化学结果还显示作为软骨细胞的主要蛋白质标记物的II型胶原的产生增加。
■我们观察到双醋瑞因单独可以启动和增强软骨形成,它除了具有抑制IL-1β的能力外,还可以用作干细胞向软骨细胞的分化因子。知道双醋瑞因的实际功能,它可能是一个很好的候选治疗骨关节炎。
UNASSIGNED: Tissue engineering is the application system that tries to restore damaged tissues by different approaches, such as cellular therapy, application of cell differential factors, and various materials. One of the important goals in tissue engineering is to guide stem cells directly to the desired tissue, and researchers tried to utilize different molecules as effective factors to improve this technique.
UNASSIGNED: This study aims to demonstrate the effects of
diacerein, a slow-acting drug for the treatment of osteoarthritis, on mesenchymal stem cell proliferation and evaluate its potential in the chondrogenesis process.
UNASSIGNED: Stem cells were isolated from adipose tissue, characterized by flow cytometry, and cells were treated with 10-5M
diacerein for three weeks. Chondrogenic gene expression of SOX9, COL2A1, ACAN, and TGFB1 were analyzed by qRT-PCR and immunocytochemistry techniques.
UNASSIGNED: Our results showed that
diacerein increased the expression of the following genes involved in chondrogenesis: SOX9 (2.9-fold, P < 0.00), COL2A1 (2.2-fold, P < 0.00), ACAN (2.7-fold, P < 0.00), and TGFB1 (2.6-fold, P < 0.00). Immunocytochemistry results also showed increased production of collagen type II as the main protein marker for chondrocytes.
UNASSIGNED: We observed that
diacerein alone could initiate and enhance chondrogenesis, and it can be used as a differentiation factor for stem cells to chondrocyte besides its ability to inhibit IL-1β. Knowing the actual function of
diacerein, it could be a good candidate for the treatment of osteoarthritis.