背景:在印度的传统医学体系中,据说最小圣殿对hriddaurbalya(影响心灵的问题)有补救作用,aakshepayuktavikara(神经障碍)和shirooga(头部疾病)。因此,在阿育吠陀实践中,它被广泛用作抗应激药物。众所周知,压力会影响功能上重要的大脑区域的神经元,例如黑质。然而,缺乏实验证据表明其对黑质神经元形态的影响。此外,目前尚不清楚O.cantum治疗是否能减轻应激诱导的黑质神经元结构变化。
目的:了解应激对大鼠黑质神经元形态的影响以及O.cantum鲜叶提取物(OSE)对应激大鼠黑质神经元的影响。
方法:本研究包括三个实验。实验一:研究3周和6周足休克应激对大鼠的影响;实验二-研究3周OSE治疗对3周应激大鼠和3周应激大鼠的影响;实验三-研究6周OSE治疗对6周应激大鼠和6周应激大鼠的影响。
结果:在实验I中,应激对黑质神经元树突乔化有显著的有害作用。实验II和III显示了在2ml和4mlOSE处理组中防止和减轻应激诱导的黑质神经元树突萎缩。OSE的保护作用在长期治疗的大鼠中更明显。
结论:足休克应激诱导大鼠黑质神经元损伤。用新鲜的O.cantum叶提取物治疗可以预防和减轻足休克应激引起的行为缺陷和黑质神经元损伤。
BACKGROUND: In Ayurveda; an Indian system of traditional medicine, Ocimum sanctum is said to have remedial effect on hriddaurbalya (problems affecting the mind), aakshepayukta vikara (nervous disorders) and shiroroga (diseases of head). Hence, in Ayurvedic practice, it is profoundly used as an antistress medicine. Stress is known to affect neurons of functionally significant brain regions like substantia nigra. However, experimental evidence showing its effect on morphology of substantia nigral neurons is lacking. In addition, whether the O. sanctum treatment attenuates stress induced substantia nigral neuronal structural changes is not known.
OBJECTIVE: To know the effect of stress on morphology of substantia nigral neurons and the effect of O. sanctum fresh leaf extract (OSE) on substantia nigral neurons of stressed rats.
METHODS: Present study included three experiments. Experiment I: To study the effect of 3 and 6 weeks of foot shock stress in rats; Experiment II- To study the effect of 3 weeks of OSE treatment on 3 week-stress undergoing rats and on 3 week-stressed rats; Experiment III- To study the effect of 6 weeks of OSE treatment in 6 week-stress undergoing rats and in 6 week-stressed rats.
RESULTS: In experiment I, stress had significant deleterious effect on dendritic arborization of substantia nigral neurons. Experiments II and III showed prevention and attenuation of the stress induced dendritic atrophy of substantia nigral neurons in both 2 ml and 4 ml OSE treatment groups. Protective effect of OSE was more pronounced in rats which are treated for a longer duration.
CONCLUSIONS: Foot shock stress induces neuronal damage in the substantia nigra of rats. Treatment with fresh leaf extract of O. sanctum could prevent and attenuate the foot shock stress induced behavioral deficit and substantia nigral neuronal damage.