In sub-Saharan Africa, acute-onset severe malaria anaemia (SMA) is a critical challenge, particularly affecting children under five. The acute drop in haematocrit in SMA is thought to be driven by an increased phagocytotic pathological process in the spleen, leading to the presence of distinct red blood cells (RBCs) with altered morphological characteristics. We hypothesized that these RBCs could be detected systematically and at scale in peripheral blood films (PBFs) by harnessing the capabilities of deep learning models. Assessment of PBFs by a microscopist does not scale for this task and is subject to variability. Here we introduce a deep learning model, leveraging a weakly supervised Multiple Instance Learning framework, to Identify SMA (MILISMA) through the presence of morphologically changed RBCs. MILISMA achieved a classification accuracy of 83% (receiver operating characteristic area under the curve [AUC] of 87%; precision-recall AUC of 76%). More importantly, MILISMA\'s capabilities extend to identifying statistically significant morphological distinctions (p < 0.01) in RBCs descriptors. Our findings are enriched by visual analyses, which underscore the unique morphological features of SMA-affected RBCs when compared to non-SMA cells. This model aided detection and characterization of RBC alterations could enhance the understanding of SMA\'s pathology and refine SMA diagnostic and prognostic evaluation processes at scale.

This study outlines a method for using surveillance cameras and an algorithm that calls a deep learning model to generate video segments featuring salmon and trout in small streams. This automated process greatly reduces the need for human intervention in video surveillance. Furthermore, a comprehensive guide is provided on setting up and configuring surveillance equipment, along with instructions on training a deep learning model tailored to specific requirements. Access to video data and knowledge about deep learning models makes monitoring of trout and salmon dynamic and hands-on, as the collected data can be used to train and further improve deep learning models. Hopefully, this setup will encourage fisheries managers to conduct more monitoring as the equipment is relatively cheap compared with customized solutions for fish monitoring. To make effective use of the data, natural markings of the camera-captured fish can be used for individual identification. While the automated process greatly reduces the need for human intervention in video surveillance and speeds up the initial sorting and detection of fish, the manual identification of individual fish based on natural markings still requires human effort and involvement. Individual encounter data hold many potential applications, such as capture-recapture and relative abundance models, and for evaluating fish passages in streams with hydropower by spatial recaptures, that is, the same individual identified at different locations. There is much to gain by using this technique as camera captures are the better option for the fish\'s welfare and are less time-consuming compared with physical captures and tagging.

Protein structure determination has made progress with the aid of deep learning models, enabling the prediction of protein folding from protein sequences. However, obtaining accurate predictions becomes essential in certain cases where the protein structure remains undescribed. This is particularly challenging when dealing with rare, diverse structures and complex sample preparation. Different metrics assess prediction reliability and offer insights into result strength, providing a comprehensive understanding of protein structure by combining different models. In a previous study, two proteins named ARM58 and ARM56 were investigated. These proteins contain four domains of unknown function and are present in Leishmania spp. ARM refers to an antimony resistance marker. The study\'s main objective is to assess the accuracy of the model\'s predictions, thereby providing insights into the complexities and supporting metrics underlying these findings. The analysis also extends to the comparison of predictions obtained from other species and organisms. Notably, one of these proteins shares an ortholog with Trypanosoma cruzi and Trypanosoma brucei, leading further significance to our analysis. This attempt underscored the importance of evaluating the diverse outputs from deep learning models, facilitating comparisons across different organisms and proteins. This becomes particularly pertinent in cases where no previous structural information is available.

The primary challenges in tea production under multiple stress exposures have negatively affected its global market sustainability, so introducing an infield fast technique for monitoring tea leaves\' stresses has tremendous urgent needs. Therefore, this study aimed to propose an efficient method for the detection of stress symptoms based on a portable smartphone with deep learning models. Firstly, a database containing over 10,000 images of tea garden canopies in complex natural scenes was developed, which included healthy (no stress) and three types of stress (tea anthracnose (TA), tea blister blight (TB) and sunburn (SB)). Then, YOLOv5m and YOLOv8m algorithms were adapted to discriminate the four types of stress symptoms; where the YOLOv8m algorithm achieved better performance in the identification of healthy leaves (98%), TA (92.0%), TB (68.4%) and SB (75.5%). Furthermore, the YOLOv8m algorithm was used to construct a model for differentiation of disease severity of TA, and a satisfactory result was obtained with the accuracy of mild, moderate, and severe TA infections were 94%, 96%, and 91%, respectively. Besides, we found that CNN kernels of YOLOv8m could efficiently extract the texture characteristics of the images at layer 2, and these characteristics can clearly distinguish different types of stress symptoms. This makes great contributions to the YOLOv8m model to achieve high-precision differentiation of four types of stress symptoms. In conclusion, our study provided an effective system to achieve low-cost, high-precision, fast, and infield diagnosis of tea stress symptoms in complex natural scenes based on smartphone and deep learning algorithms.

This research delves into the exploration of the potential of tocopherol-based nanoemulsion as a therapeutic agent for cardiovascular diseases (CVD) through an in-depth molecular docking analysis. The study focuses on elucidating the molecular interactions between tocopherol and seven key proteins (1O8a, 4YAY, 4DLI, 1HW9, 2YCW, 1BO9 and 1CX2) that play pivotal roles in CVD development. Through rigorous in silico docking investigations, assessment was conducted on the binding affinities, inhibitory potentials and interaction patterns of tocopherol with these target proteins. The findings revealed significant interactions, particularly with 4YAY, displaying a robust binding energy of -6.39 kcal/mol and a promising Ki value of 20.84 μM. Notable interactions were also observed with 1HW9, 4DLI, 2YCW and 1CX2, further indicating tocopherol\'s potential therapeutic relevance. In contrast, no interaction was observed with 1BO9. Furthermore, an examination of the common residues of 4YAY bound to tocopherol was carried out, highlighting key intermolecular hydrophobic bonds that contribute to the interaction\'s stability. Tocopherol complies with pharmacokinetics (Lipinski\'s and Veber\'s) rules for oral bioavailability and proves safety non-toxic and non-carcinogenic. Thus, deep learning-based protein language models ESM1-b and ProtT5 were leveraged for input encodings to predict interaction sites between the 4YAY protein and tocopherol. Hence, highly accurate predictions of these critical protein-ligand interactions were achieved. This study not only advances the understanding of these interactions but also highlights deep learning\'s immense potential in molecular biology and drug discovery. It underscores tocopherol\'s promise as a cardiovascular disease management candidate, shedding light on its molecular interactions and compatibility with biomolecule-like characteristics.

Clinical Decision Support Systems (CDSS) are essential tools in contemporary healthcare, enhancing clinicians\' decisions and patient outcomes. The integration of artificial intelligence (AI) is now revolutionizing CDSS even further. This review delves into AI technologies transforming CDSS, their applications in healthcare decision-making, associated challenges, and the potential trajectory toward fully realizing AI-CDSS\'s potential. The review begins by laying the groundwork with a definition of CDSS and its function within the healthcare field. It then highlights the increasingly significant role that AI is playing in enhancing CDSS effectiveness and efficiency, underlining its evolving prominence in shaping healthcare practices. It examines the integration of AI technologies into CDSS, including machine learning algorithms like neural networks and decision trees, natural language processing, and deep learning. It also addresses the challenges associated with AI integration, such as interpretability and bias. We then shift to AI applications within CDSS, with real-life examples of AI-driven diagnostics, personalized treatment recommendations, risk prediction, early intervention, and AI-assisted clinical documentation. The review emphasizes user-centered design in AI-CDSS integration, addressing usability, trust, workflow, and ethical and legal considerations. It acknowledges prevailing obstacles and suggests strategies for successful AI-CDSS adoption, highlighting the need for workflow alignment and interdisciplinary collaboration. The review concludes by summarizing key findings, underscoring AI\'s transformative potential in CDSS, and advocating for continued research and innovation. It emphasizes the need for collaborative efforts to realize a future where AI-powered CDSS optimizes healthcare delivery and improves patient outcomes.

BACKGROUND: The serotonergic system modulates brain processes via functionally distinct subpopulations of neurons with heterogeneous properties, including their electrophysiological activity. In extracellular recordings, serotonergic neurons to be investigated for their functional properties are commonly identified on the basis of \"typical\" features of their activity, i.e. slow regular firing and relatively long duration of action potentials. Thus, due to the lack of equally robust criteria for discriminating serotonergic neurons with \"atypical\" features from non-serotonergic cells, the physiological relevance of the diversity of serotonergic neuron activities results largely understudied.
METHODS: We propose deep learning models capable of discriminating typical and atypical serotonergic neurons from non-serotonergic cells with high accuracy. The research utilized electrophysiological in vitro recordings from serotonergic neurons identified by the expression of fluorescent proteins specific to the serotonergic system and non-serotonergic cells. These recordings formed the basis of the training, validation, and testing data for the deep learning models. The study employed convolutional neural networks (CNNs), known for their efficiency in pattern recognition, to classify neurons based on the specific characteristics of their action potentials.
RESULTS: The models were trained on a dataset comprising 27,108 original action potential samples, alongside an extensive set of 12 million synthetic action potential samples, designed to mitigate the risk of overfitting the background noise in the recordings, a potential source of bias. Results show that the models achieved high accuracy and were further validated on \"non-homogeneous\" data, i.e., data unknown to the model and collected on different days from those used for the training of the model, to confirm their robustness and reliability in real-world experimental conditions.
METHODS: Conventional methods for identifying serotonergic neurons allow recognition of serotonergic neurons defined as typical. Our model based on the analysis of the sole action potential reliably recognizes over 94% of serotonergic neurons including those with atypical features of spike and activity.
CONCLUSIONS: The model is ready for use in experiments conducted with the here described recording parameters. We release the codes and procedures allowing to adapt the model to different acquisition parameters or for identification of other classes of spontaneously active neurons.

Ensuring the safety and efficacy of chemical compounds is crucial in small-molecule drug development. In the later stages of drug development, toxic compounds pose a significant challenge, losing valuable resources and time. Early and accurate prediction of compound toxicity using deep learning models offers a promising solution to mitigate these risks during drug discovery. In this study, we present the development of several deep-learning models aimed at evaluating different types of compound toxicity, including acute toxicity, carcinogenicity, hERG_cardiotoxicity (the human ether-a-go-go related gene caused cardiotoxicity), hepatotoxicity, and mutagenicity. To address the inherent variations in data size, label type, and distribution across different types of toxicity, we employed diverse training strategies. Our first approach involved utilizing a graph convolutional network (GCN) regression model to predict acute toxicity, which achieved notable performance with Pearson R 0.76, 0.74, and 0.65 for intraperitoneal, intravenous, and oral administration routes, respectively. Furthermore, we trained multiple GCN binary classification models, each tailored to a specific type of toxicity. These models exhibited high area under the curve (AUC) scores, with an impressive AUC of 0.69, 0.77, 0.88, and 0.79 for predicting carcinogenicity, hERG_cardiotoxicity, mutagenicity, and hepatotoxicity, respectively. Additionally, we have used the approved drug dataset to determine the appropriate threshold value for the prediction score in model usage. We integrated these models into a virtual screening pipeline to assess their effectiveness in identifying potential low-toxicity drug candidates. Our findings indicate that this deep learning approach has the potential to significantly reduce the cost and risk associated with drug development by expediting the selection of compounds with low toxicity profiles. Therefore, the models developed in this study hold promise as critical tools for early drug candidate screening and selection.

The prediction of suspended sediment load (SSL) within riverine systems is critical to understanding the watershed\'s hydrology. Therefore, the novelty of our research is developing an interpretable (explainable) model based on deep learning (DL) and Shapley Additive ExPlanations (SHAP) interpretation technique for prediction of SSL in the riverine systems. This paper investigates the abilities of four DL models, including dense deep neural networks (DDNN), long short-term memory (LSTM), gated recurrent unit (GRU), and simple recurrent neural network (RNN) models for the prediction of daily SSL using river discharge and rainfall data at a daily time scale in the Taleghan River watershed, northwestern Tehran, Iran. The performance of models was evaluated by using several quantitative and graphical criteria. The effect of parameter settings on the performance of deep models on SSL prediction was also investigated. The optimal optimization algorithms, maximum iteration (MI), and batch size (BC) were obtained for modeling daily SSL, and structure of the model impact on prediction remarkably. The comparison of prediction accuracy of the models illustrated that DDNN (with R2 = 0.96, RMSE = 333.46) outperformed LSTM (R2 = 0.75, RMSE = 786.20), GRU (R2 = 0.73, RMSE = 825.67), and simple RNN (R2 = 0.78, RMSE = 741.45). Furthermore, the Taylor diagram confirmed that DDNN has the highest performance among other models. Interpretation techniques can address the black-box nature of models, and here, SHAP was applied to develop an interpretable DL model to interpret of DL model\'s output. The results of SHAP showed that river discharge has the strongest impact on the model\'s output in estimating SSL. Overall, we conclude that DL models have great potential in watersheds to predict SSL. Therefore, different interpretation techniques as tools to interpret DL model\'s output (DL model is as black-box model) are recommended in future research.

Elderly falls are a major concerning threat resulting in over 1.5-2 million elderly people experiencing severe injuries and 1 million deaths yearly. Falls experienced by Elderly people may lead to a long-term negative impact on their physical and psychological health conditions. Major healthcare research had focused on this lately to detect and prevent the fall. In this work, an Artificial Intelligence (AI) edge computing based wearable device is designed and developed for detection and prevention of fall of elderly people. Further, the various deep learning algorithms such as Convolutional Neural Network (CNN), Recurrent Neural Network (RNN), Long Short-Term Memory (LSTM), Gated Recurrent Unit (GRU) are utilized for activity recognition of elderly. Also, the CNN-LSTM, RNN-LSTM and GRU-LSTM with and without attention layer respectively are utilized and the performance metrics are analyzed to find the best deep learning model. Furthermore, the three different hardware boards such as Jetson Nano developer board, Raspberry PI 3 and 4 are utilized as an AI edge computing device and the best deep learning model is implemented and the computation time is evaluated. Results demonstrate that the CNN-LSTM with attention layer exhibits the accuracy, recall, precision and F1_Score of 97%, 98%, 98% and 0.98 respectively which is better when compared to other deep learning models. Also, the computation time of NVIDIA Jetson Nano is less when compared to other edge computing devices. This work appears to be of high societal relevance since the proposed wearable device can be used to monitor the activity of elderly and prevents the elderly falls which improve the quality of life of elderly people.