在食品营养安全领域,富含矿物质的谷物的发展在对抗营养不良中起着关键作用。在目前的调查范围内,我们努力辨别对印度谷子中谷物铁积累的改善负责的成绩单。这种追求需要在穗发育-穗出现和挤奶阶段的两个不同阶段对基因型BAR-1433(高Fe含量)和BAR-1423(低Fe含量)进行转录组测序。在尖峰出现的背景下,我们确定了一组895个上调转录本和126个下调转录本,这些转录本描述了高和低籽粒铁基因型之间的差异.相比之下,在挤奶阶段,上调的转录本总数达到436份,而下调的转录本总数为285份.在两个发育阶段始终上升的转录本经过了功能注释,将它们的作用与核仁蛋白对齐,金属-烟胺转运蛋白,核糖核蛋白复合物,长春碱合酶,纤维素合酶,生长素响应因子,胚胎发生丰富的蛋白质,细胞色素c氧化酶,和含锌指BED结构域的蛋白质。同时,转录物的异质谱在整个不同阶段表现出差异表达和上调。这些转录本涵盖了各个方面,如ABC转运蛋白家族蛋白,钙依赖性激酶家族,铁蛋白,金属离子结合,铁硫簇结合,细胞色素家族,锌指转录因子家族,铁氧还蛋白-NADP还原酶1型家族,推定漆酶,Multicopper氧化酶家族,和萜烯合酶家族。为了验证这些转录本的可靠性,六个重叠群代表可能的函数,包括金属运输机,铁硫配位,金属离子结合,生长素响应性GH3样蛋白2和细胞色素P45071B16用于引物设计。随后,这些引物通过qRT-PCR在验证过程中使用,结果与转录组结果一致。这项研究记录了一系列与谷子中铁含量升高有关的基因,展示了在标记辅助选择中利用转录组见解用铁强化谷子的概念证明。这标志着首次全面的转录组分析,描绘了在谷子范式中的穗部发育阶段与不同水平的谷物铁含量相关的转录本。
In the realm of food nutritional security, the development of mineral-rich grains assumes a pivotal role in combating malnutrition. Within the scope of the current investigation, we endeavoured to discern the transcripts accountable for the improved accumulation of grain-Fe within Indian barnyard millet. This pursuit entailed transcriptome sequencing of genotypes BAR-1433 (with high Fe content) and BAR-1423 (with low Fe content) during two distinct stages of spike development-spike emergence and milking stage. In the context of spike emergence, we identified a cohort of 895 up-regulated transcripts and 126 down-regulated transcripts that delineated the difference between the high and low grain-Fe genotypes. In contrast, during the milking stage, the tally of up-regulated transcripts reached 436, while down-regulated transcripts numbered 285. The transcripts that consistently ascended in both developmental stages underwent functional annotation, aligning their roles with nucleolar proteins, metal-nicotianamine transporters, ribonucleoprotein complexes, vinorine synthases, cellulose synthases, auxin response factors, embryogenesis abundant proteins, cytochrome c oxidases, and zinc finger BED domain-containing proteins. Meanwhile, a heterogeneous spectrum of transcripts exhibited differential expression and upregulation throughout the distinct stages. These transcripts encompassed various facets, such as ABC Transporter family proteins, Calcium-dependent kinase family, Ferritin, Metal ion binding, Iron-sulfur cluster binding, Cytochrome family, Zinc finger transcription factor family, Ferredoxin-NADP reductase type 1 family, Putative laccase, Multicopper oxidase family, and Terpene synthase family. To authenticate the reliability of these transcripts, six contigs representing probable functions, including metal transporters, iron sulfur coordination, metal ion binding, auxin-responsive GH3-like protein 2, and cytochrome P450 71B16, were harnessed for primer design. Subsequently, these primers were utilized in the validation process through qRT-PCR, with the outcomes aligning harmoniously with the transcriptome results. This study chronicles a constellation of genes linked to elevated iron content within barnyard millet, showcasing a proof of concept for leveraging transcriptome insights in marker-assisted selection to fortify barnyard millet with iron. This marks the inaugural comprehensive transcriptome analysis delineating transcripts associated with varying levels of grain-iron content during the panicle developmental stages within the barnyard millet paradigm.