这项研究的目的是研究一种新形式的生物素(生物素镁)对血清葡萄糖的影响,血脂谱,和大鼠肝脏脂质代谢相关蛋白水平。将42只大鼠分为六组,并饲喂以标准饮食为基础的蛋清粉末饮食,并补充了0.01、1或100mg/kg体重的d-生物素或0.01、1或100mg/kg体重的生物素镁,持续35天。两种形式的生物素都不影响(p>0.05)血清葡萄糖或胰岛素浓度。血清总胆固醇和甘油三酯与来自两种来源的生物素一起降低(p<0.05)。当比较1mg/kg剂量时,使用生物素镁的浓度较低(p<0.05)。血清,肝脏,和脑生物素和肝脏环磷酸鸟苷(cGMP)浓度更大,当大鼠用生物素镁治疗与d-生物素,特别是当比较1和100mg/kg剂量组时(p<0.05)。两种生物素形式均降低肝脏SREBP-1c和FAS,并增加AMPK-α1,ACC-1,ACC-2,PCC,和MCC水平(p<0.05)。生物素镁更强调了反应的幅度。生物素镁,与商业D-生物素相比,在降低血清脂质浓度和调节脂质代谢相关生物标志物的蛋白质水平方面更有效。
The objective of this study was to investigate the effects of a novel form of biotin (magnesium biotinate) on serum glucose, lipid profile, and hepatic lipid metabolism-related protein levels in rats. Forty-two rats were divided into six groups and fed a standard diet-based egg white powdered diet supplemented with either d-biotin at 0.01, 1, or 100 mg/kg BW or magnesium biotinate at 0.01, 1, or 100 mg/kg BW for 35 days. Neither form of biotin influenced (p > 0.05) serum glucose or insulin concentrations. Serum total cholesterol and triglyceride decreased with biotin from both sources (p < 0.05). Concentrations were lower with magnesium biotinate when comparing the 1 mg/kg dose (p < 0.05). Serum, liver, and brain biotin and liver cyclic guanosine monophosphate (cGMP) concentrations were greater when rats were treated with magnesium biotinate versus d-biotin, particularly when comparing the 1 and 100 mg/kg dose groups (p < 0.05). Both biotin forms decreased the liver SREBP-1c and FAS and increased AMPK-α1, ACC-1, ACC-2, PCC, and MCC levels (p < 0.05). The magnitudes of responses were more emphasized with magnesium biotinate. Magnesium biotinate, compared with a commercial d-biotin, is more effective in reducing serum lipid concentrations and regulating protein levels of lipid metabolism-related biomarkers.