背景:行为,社会,身体特征是为了区分性别,然而,关于大脑转录水平性别差异的研究是有限的。这里,我们调查了青春期前食蟹猴的性发散性脑转录组学,人类常用的替代物种。方法:使用RNA测序生成颞叶的转录组学图谱,腹侧中脑,先前用腺相关病毒载体混合物处理的3只雌性和3只雄性食蟹猴的小脑。使用DeSeq2进行统计分析以确定所有三个叶中差异表达的蛋白质编码基因,错误发现率校正P值为.05。结果:我们确定了颞叶中的靶基因,腹侧中脑,和小脑具有翻译功能,豁免权,行为,和表现出统计学上显着的性别差异表达的神经系统疾病。结论:我们提供了关于心理健康和传染病的两性之间观察到的流行病学差异的潜在机制见解,比如COVID19。我们的结果提供了有关非人类灵长类动物脑转录组学中性别差异的青春期前信息,并可能提供有关人类生物性别之间健康差异的见解。
UNASSIGNED: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrogate species to humans.
UNASSIGNED: A transcriptomic profile using RNA sequencing was generated for the temporal lobe, ventral midbrain, and cerebellum of 3 female and 3 male cynomolgus macaques previously treated with an Adeno-associated virus vector mix. Statistical analyses to determine differentially expressed protein-coding genes in all three lobes were conducted using DeSeq2 with a false discovery rate corrected P value of .05.
UNASSIGNED: We identified target genes in the temporal lobe, ventral midbrain, and cerebellum with functions in translation, immunity, behavior, and neurological disorders that exhibited statistically significant sexually divergent expression.
UNASSIGNED: We provide potential mechanistic insights to the epidemiological differences observed between the sexes with regards to mental health and infectious diseases, such as COVID19. Our results provide pre-pubertal information on sexual differences in non-human primate brain transcriptomics and may provide insight to health disparities between the biological sexes in humans.