目的:评价caspase-3、细胞色素c和热休克蛋白70(Hsp70)在卵巢储备正常(NOR)和卵巢储备功能减退(DOR)妇女卵胞浆内单精子注射(ICSI)颗粒细胞(GCs)中的表达。
方法:在取卵过程中收集了117名不育妇女的GCs。将患者分为以下四组:DOR-COC评分为0,DOR-COC评分为I,NOR-COC评分为0,NOR-COC评分为I。caspase-3,细胞色素c,在GC中进行免疫组织化学和Hsp70分析。ICSI结果进行了前瞻性评估。
结果:DOR-COC评分I组(15,30.6%;14,38.9%),NOR-COC评分I组(19,38.77%;19,52.7%),与DOR-COC评分0(12,24.4%;3,6.1%)和NOR-COC评分0(3,6.1%;0%)组(p=0.0001;0.00002)相比,分别。在DOR-COC评分为0组(23,65.7%;25,71.4%)和NOR-COC评分为0组(19,61.3%;20,64.5%)中,Caspase-3和细胞色素c表达水平较高,与I组的DOR-COC评分(8,32%;9,36%)和I组的NOR-COC评分(7,26.9%;8,30.8%)相比(p=0.00297;p=0.002),分别。在DOR-COC评分为0(11,31.4%)和NOR-COC评分为0(10,32.3%)组,Hsp70表达水平较低,与I组的DOR-COC评分(16,64%)和I组的NOR-COC评分(20,76.9%)相比(p=0.001),分别。DOR组Hsp70表达水平与第3天优质胚胎数呈正相关,与雌二醇水平呈负相关。
结论:我们的数据表明,COC评分为0与凋亡蛋白的表达水平增加有关,抗凋亡蛋白表达水平降低,有和没有DOR的女性的ICSI临床结局较差。
OBJECTIVE: To evaluate the expressions of caspase-3 and cytochrome c and heat shock protein 70 (Hsp70) in granulosa cells (GCs) from women with normal ovarian reserve (NOR) and diminished ovarian reserve (DOR) undergoing intracytoplasmic sperm injection (ICSI).
METHODS: GCs were collected from 117 infertile women during oocyte retrieval. Patients were classified into four groups as follows: DOR-COC score of 0, DOR-COC score of I, NOR-COC score of 0, and NOR-COC score of I. The caspase-3, cytochrome c, and Hsp70 analyses were performed immunohistochemically in GCs. The ICSI outcomes were evaluated prospectively.
RESULTS: The clinical pregnancy and live birth rates were higher in DOR-COC score of I (15, 30.6%; 14, 38.9%) and NOR-COC score of I (19, 38.77%; 19, 52.7%) groups, compared with DOR-COC score of 0 (12, 24.4%; 3, 6.1%) and NOR-COC score of 0 (3, 6.1%; 0%) groups (p = 0.0001; 0.00002), respectively. Caspase-3 and cytochrome c expression levels were higher in DOR-COC score of 0 (23, 65.7%; 25, 71.4%) and NOR-COC score of 0 groups (19, 61.3%; 20, 64.5%), compared with DOR-COC score of I (8, 32%; 9, 36%) and NOR-COC score of I groups (7, 26.9%; 8, 30.8%) (p = 0.00297; p = 0.002), respectively. Lower expression levels of Hsp70 were found in DOR-COC score of 0 (11, 31.4%) and NOR-COC score of 0 groups (10, 32.3%), compared with DOR-COC score of I (16, 64%) and NOR-COC score of I groups (20, 76.9%) (p = 0.001), respectively. Hsp70 expression levels were positively correlated with the number of day 3 good-quality embryo and negatively correlated with estradiol levels in the DOR group.
CONCLUSIONS: Our data suggest that COC score of 0 is associated with increased expression levels of apoptotic proteins, decreased expression levels of anti-apoptotic protein, and poor ICSI clinical outcomes in women with and without DOR.