睾酮酯是通常用于确认变性人性别认同的激素。本研究评估了接受跨性别激素治疗的动物模型中睾丸激素对肾脏形态和功能的影响。将两个月龄的Wistar大鼠分为三组:雄性对照组(MC),女性控制(FC),和女性的睾丸激素治疗(FTT)。FTT组接受cyponate睾酮(3.0mg/kg,i.m.),MC和MF组每10天接受一次车辆油,为期4个月。在6月龄时评估肾功能和间接收缩压(SBP)测量值。血浆和尿液中尿素的浓度,肌酐,钠,钾,渗透压,测量肾小球滤过率(GFR)。对肾脏称重,石蜡包埋,并准备组织学切片以评估肾小球面积。我们核实了FTT组,与FC相比,增加了肾脏重量[MC,3.2±0.05;FC,1.8±0.04;FTT,2.2±0.06;g],尿液渗透压降低[MC,486.9±18.3;FC,1012.0±5.4;FTT,768.2±40.3mOsm/L/gkw],降低GFR[MC,0.77±0.04;FC,0.78±0.02;FTT,0.67±0.03;mL/min/gkw],更大的肾小球面积[MC,9334±120.8;FC,7884±112.8;FTT,9078±133.4µm2],和更高的SBP[MC,126±3.4;FC,119±1.0;FTT,131±1.4;mmHg]。与MC相比,FC和FTT的钠排泄更高[MC,0.34±0.05;FC,0.56±0.06;FTT,0.54±0.04;mEq/24h/gkw]。在雌性大鼠中使用睾丸激素进行跨性激素治疗会引起肾脏形态功能变化,并且可能是收缩压升高的基础。这表明长期睾酮治疗的适应性与在变性人中观察到的适应性相似。
Testosterone esters are hormones commonly used for affirming gender identity in transmen. The present study evaluates the effect of testosterone on renal morphology and function in an animal model submitted to cross-sex hormone therapy used for transmen. Two-month-old Wistar rats were divided into three groups: male control (MC), female control (FC), and female on testosterone therapy (FTT). The FTT group received testosterone cypionate (3.0 mg/kg, i.m.), and the MC and MF groups received vehicle oil every 10 days for 4 months. Renal function and indirect systolic blood pressure (SBP) measurements were evaluated at 6 months of age. Plasma and urine concentrations of urea, creatinine, sodium, potassium, osmolality, and glomerular filtration rate (GFR) were measured. The kidneys were weighed, paraffin-embedded, and histological sections were prepared to evaluate the glomerular area. We verified that the FTT group, in comparison to FC, had increased kidney weight [MC, 3.2 ± 0.05; FC, 1.8 ± 0.04; FTT, 2.2 ± 0.06; g], decreased urine osmolarity [MC, 486.9 ± 18.3; FC, 1012.0 ± 5.4; FTT, 768.2 ± 40.3 mOsm/L/g kw], reduced GFR [MC, 0.77 ± 0.04; FC, 0.78 ± 0.02; FTT, 0.67 ± 0.03; mL/min/g kw], larger glomerular area [MC, 9334 ± 120.8; FC, 7884 ± 112.8; FTT, 9078 ± 133.4 µm2 ], and higher SBP [MC, 126 ± 3.4; FC, 119 ± 1.0; FTT, 131 ± 1.4; mmHg]. Sodium excretion was higher in FC and FTT in comparison to MC [MC, 0.34 ± 0.05; FC, 0.56 ± 0.06; FTT, 0.54 ± 0.04; mEq/24 h/g kw]. Cross-sex hormone therapy with testosterone in female rats induces renal morphofunctional changes and may underlie increased systolic pressure, suggesting an adaptation similar to what is observed in transmen on long-term testosterone therapy.