柑橘是世界上最重要的水果作物之一,和根相关的微生物群可以对树木的健康和生长产生深远的影响。
■在共同努力中,国际柑橘微生物组联盟从六大洲的九个柑橘生产国收集了样本,调查了全球柑橘根部微生物群。我们分析了16SrDNA和ITS2扩增子测序数据,以鉴定柑橘根样品中主要的原核和真菌分类群。在与根相关的微生物群落与相应的根际和大块土壤样品中的微生物群落之间进行了比较分析。此外,我们进行了基于基因型的分组比较,以评估柑橘基因型对根系微生物组成的影响.
■十个主要的原核生物门,含有9个细菌门,包括变形杆菌,放线菌,酸杆菌,和拟杆菌和一个古细菌门(Thaumarchaoota),在柑橘根样品中鉴定出多种真菌门,包括子囊菌和担子菌。与来自相同树木的相应根际和大块土壤样品的微生物群落相比,与根际相比,根中的原核和真菌群落表现出更低的多样性和复杂性,但模块化更大。总的来说,在细菌群落中鉴定出30个富含根的属和150个贫根的属,而富集了21个真菌属,与根际相比,根生态位减少了147个真菌属。柑橘基因型显著影响根系原核和真菌群落。此外,我们已经确定了包含酸性杆菌的核心根原核属,异型根瘤菌,缓生根瘤菌,Chitinophaga,Cupriavidus,Devosia,Dongia,Niastella,假单胞菌,鞘氨醇,类固醇和链霉菌,和核心真菌属,包括Acroalymma,枝孢霉,镰刀菌,赤霉素,被孢霉,新孢霉和卷肠。预测了这些根微生物群核心属的潜在功能。
■总的来说,这项研究为微生物群落的组装提供了新的见解,并在广泛的地理范围内确定了柑橘根微生物群的核心成员。这些发现为操纵根部微生物区以增强植物生长和健康提供了有价值的信息。
UNASSIGNED: Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.
UNASSIGNED: In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples. Comparative analyses were conducted between root-associated microbial communities and those from the corresponding rhizosphere and bulk soil samples. Additionally, genotype-based group-wise comparisons were performed to assess the impact of citrus genotype on root microbiota composition.
UNASSIGNED: Ten predominant prokaryotic phyla, containing nine bacterial phyla including Proteobacteria, Actinobacteria, Acidobacteria, and Bacteroidetes and one archaeal phylum (Thaumarchaeota), and multiple fungal phyla including Ascomycota and Basidiomycota were identified in the citrus root samples. Compared with the microbial communities from the corresponding rhizosphere and bulk soil samples from the same trees, the prokaryotic and fungal communities in the roots exhibited lower diversity and complexity but greater modularity compared to those in the rhizosphere. In total, 30 root-enriched and 150 root-depleted genera in bacterial community were identified, whereas 21 fungal genera were enriched, and 147 fungal genera were depleted in the root niche compared with the rhizosphere. The citrus genotype significantly affected the root prokaryotic and fungal communities. In addition, we have identified the core root prokaryotic genera comprising Acidibacter, Allorhizobium, Bradyrhizobium, Chitinophaga, Cupriavidus, Devosia, Dongia, Niastella, Pseudomonas, Sphingobium, Steroidobacter and Streptomyces, and the core fungal genera including Acrocalymma, Cladosporium, Fusarium, Gibberella, Mortierella, Neocosmospora and Volutella. The potential functions of these core genera of root microbiota were predicted.
UNASSIGNED: Overall, this study provides new insights into the assembly of microbial communities and identifies core members of citrus root microbiota across a wide geographic range. The findings offer valuable information for manipulating root microbiota to enhance plant growth and health.