■淋病是一个主要的公共卫生问题。随着全球对最后一线抗生素治疗方案的耐药性的出现和传播,淋病有可能在未来无法治愈。因此,这项研究对加纳收集的淋病奈瑟菌进行了全基因组鉴定,以鉴定循环菌株的谱系以及它们的表型和基因型抗菌素耐药性(AMR)谱.
使用牛津纳米孔MinION和IlluminaMiSeq测序平台对56个分离物进行全基因组测序(WGS)。综合抗菌素耐药性数据库(CARD)和PUBMLST.org/奈瑟菌数据库用于对AMR中涉及的染色体和质粒基因进行分类。核心基因组多位点序列分型(cgMLST)方法用于比较基因组学分析。
■通过E测试方法测量的体外抗性显示100%,对四环素的抗性为91.0%和85.7%,青霉素和环丙沙星,分别。通过多位点序列分型(MLST)共鉴定出22种序列类型(STs),ST-14422(n=10),ST-1927(n=8)和ST-11210(n=7)是最普遍的。还鉴定了六种新的ST(ST-15634、15636-15639和15641)。所有分离株都具有染色体AMR决定簇,可赋予对β-内酰胺抗菌剂和四环素的抗性。一个单一的头孢克肟抗性菌株,属于淋病奈瑟菌多抗原序列型(NG-MAST)ST1407,鉴定了与广泛的头孢菌素耐药相关的类型。淋病奈瑟菌抗菌素耐药性序列分型(NG-STAR),确定了29种独特的序列类型,ST-464(n=8)和新型ST-3366(n=8)是最普遍的。值得注意的是,29个STS中有20个是小说,表明加纳菌株中分子AMR决定子的独特性质。质粒非常普遍:pTetM和pblaTEM在96%和92%的分离株中发现,分别。TEM-135等位基因,这是一种氨基酸变化,远离产生稳定的广谱β-内酰胺酶,可能导致完全的头孢菌素耐药性,在28.5%的分离株中被鉴定。使用WGS,我们对加纳的淋病奈瑟菌菌株进行了鉴定,简要介绍了该国淋球菌AMR的现状,并强调了持续进行基因组监测的必要性。
UNASSIGNED: Gonorrhoea is a major public health concern. With the global emergence and spread of resistance to last-line antibiotic treatment options, gonorrhoea threatens to be untreatable in the future. Therefore, this study performed whole genome characterization of Neisseria gonorrhoeae collected in Ghana to identify lineages of circulating strains as well as their phenotypic and genotypic antimicrobial resistance (AMR) profiles.
UNASSIGNED: Whole genome sequencing (WGS) was performed on 56 isolates using both the Oxford Nanopore MinION and Illumina MiSeq sequencing platforms. The Comprehensive Antimicrobial Resistance Database (CARD) and PUBMLST.org/neisseria databases were used to catalogue chromosomal and plasmid genes implicated in AMR. The core genome multi-locus sequence typing (cgMLST) approach was used for comparative genomics analysis.
UNASSIGNED: In vitro resistance measured by the E-test method revealed 100%, 91.0% and 85.7% resistance to tetracycline, penicillin and ciprofloxacin, respectively. A total of 22 sequence types (STs) were identified by multilocus sequence typing (MLST), with ST-14422 (n = 10), ST-1927 (n = 8) and ST-11210 (n = 7) being the most prevalent. Six novel STs were also identified (ST-15634, 15636-15639 and 15641). All isolates harboured chromosomal AMR determinants that confer resistance to beta-lactam antimicrobials and tetracycline. A single cefixime-resistant strain, that belongs to N. gonorrhoeae multiantigen sequence type (NG-MAST) ST1407, a type associated with widespread cephalosporin resistance was identified. Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR), identified 29 unique sequence types, with ST-464 (n = 8) and the novel ST-3366 (n = 8) being the most prevalent. Notably, 20 of the 29 STs were novel, indicative of the unique nature of molecular AMR determinants in the Ghanaian strains. Plasmids were highly prevalent: pTetM and pblaTEM were found in 96% and 92% of isolates, respectively. The TEM-135 allele, which is an amino acid change away from producing a stable extended-spectrum β-lactamase that could result in complete cephalosporin resistance, was identified in 28.5% of the isolates. Using WGS, we characterized N. gonorrhoeae strains from Ghana, giving a snapshot of the current state of gonococcal AMR in the country and highlighting the need for constant genomic surveillance.