目的:目的是评估黑色素瘤抗原(MAGE)A从A1到10(A1-10)和单个MAGEA家族在周围型肺肿瘤中的表达,并分析其与组织病理学发现的相关性。
方法:对67例周围型肺肿瘤样本进行了横断面研究,这些样本是通过核心活检从临床诊断为肺和纵隔肿瘤的患者中获得的。标本被分成两个,一个进行组织病理学诊断,最后一个进行mRNAMAGEA检查。使用通用引物进行巢式聚合酶链反应(PCR),MF10/MR10和MF10/MR12。通过适当的统计技术分析收集的数据。
结果:组织病理学发现41例(61.2%)标本为恶性细胞,26例(38.8%)标本为非恶性细胞。MAGEA1-10在47(70.1%)处表达,MAGEA1-6在25(37.3%)处表达。在恶性细胞中,MAGEA1-10和MAGEA1-6分别为33(80.5%)和19(46.3%),分别。在非恶性细胞中,MAGEA1-10和MAGEA1-6在14(53.9%)和6(23.1%,)分别。MAGEA1-10和MAGEA8的表达与恶性或非恶性细胞的组织病理学发现显着相关。敏感性,特异性,MAGEA1-10的诊断准确率为80.5%,46.2%,和67.2%,而MAGEA8分别为41.5%,88.5%,和59.7%,分别。
结论:MAGEA1-10的表达是最常见的,并且与组织病理学发现有关。此外,它比其他方法更敏感,更特异,诊断准确率更高.因此,MAGEA1-10检测可提高周围型肺肿瘤恶性肿瘤诊断的准确性.
OBJECTIVE: The objective was to evaluate the expression of melanoma antigen (MAGE) A from A1 to 10 (A1-10) and the individual MAGE A family in the peripheral lung tumors and to analyze its association with histopathological findings.
METHODS: A cross-sectional study was conducted on 67 samples of peripheral lung tumor obtained by core biopsies from patients with clinical diagnoses such as lung and mediastinal tumors. The specimens were divided into two, one to perform histopathological diagnosis and the last for mRNA MAGE A examination. A Nested polymerase chain reaction (PCR) was performed using universal primer, MF10/MR10 and MF10/MR12. The collected data were analyzed by appropriate statistical techniques.
RESULTS: The histopathological finding showed 41 (61.2 %) of specimens as malignant cells and 26 (38.8 %) of specimens as non-malignant cells. MAGE A1-10 was expressed at 47 (70.1 %) and MAGE A1-6 was expressed at 25 (37.3 %) of specimens. In a malignant cell, MAGE A1-10 and MAGE A1-6 were expressed at 33 (80.5 %) and 19 (46.3 %), respectively. In non-malignant cells, MAGE A1-10 and MAGE A1-6 were expressed at 14 (53.9 %) and 6 (23.1 %,) respectively. The MAGE A1-10 and MAGE A8 expressions were significantly associated with histopathological findings of malignant or non-malignant cells. The sensitivity, specificity, and diagnostic accuracy of MAGE A1-10 were 80.5 %, 46.2 %, and 67.2 %, respectively; while for MAGE A8 were 41.5 %, 88.5 %, and 59.7 %, respectively.
CONCLUSIONS: The MAGE A1-10 expression was the most commonly detected and associated with the histopathological finding. Moreover, it was more sensitive and specific and had higher diagnostic accuracy than others. Therefore, the MAGE A1-10 assay may improve the accuracy of the diagnosis of malignancy in peripheral lung tumors.