AnyplexIIHPV-28(HPV-28)可以单独检测28种HPV基因型。我们评估了线性阵列HPV基因分型(LA-HPV)和HPV-28之间的一致性,以检测410个储存的肛门生殖器样本(75个肛门样本,335个医生收集的宫颈样本)从410个人(13名男性,397名妇女),包括202名HIV血清阳性个体。393例(95.9%,95%置信区间[CI]:93.4-97.4)和382(93.2%,95%CI:90.3-95.3)HPV-28和LA-HPV样品(p=0.13),分别,对于96.3%的良好一致性(κ=0.65)。在10503例HPV分型结果中,10195(780阳性,9577阴性)是一致的,一致性为97.1%(95%CI:96.7-97.4),κ=0.82(95%CI:0.80-0.84)。27种基因型的平均类型特异性一致性为97.0%,95%CI:95.8-98.5(κ=0.86±0.07,95%CI:0.83-0.88)。对于所有高风险基因型(κ=0.81-0.97)和大多数其他基因型,除类型42、44、54、68和69外,均获得了极好的一致性。LA-HPV检测到的不一致样品中每个样品的平均类型数(3.0,95%CI:2.7-3.4)大于一致样品中的类型数(1.4,95%CI:1.3-1.5;p<0.001)。总之,HPV-28与LA-HPV相比,但更常见的是HPV42和HPV68阳性。
Anyplex II HPV-28 (HPV-28) can detect individually 28 HPV genotypes. We assessed the agreement between linear array HPV genotyping (LA-HPV) and HPV-28 for detection of 27 HPV genotypes in 410 stored anogenital samples (75 anal samples, 335 physician-collected cervical samples) collected over 5 years from 410 individuals (13 men, 397 women), including 202 HIV-seropositive individuals. HPV DNA was detected in 393 (95.9%, 95% confidence interval [CI]: 93.4-97.4) and 382 (93.2%, 95% CI: 90.3-95.3) samples with HPV-28 and LA-HPV (p = 0.13), respectively, for a good agreement of 96.3% (κ = 0.65). Of the 10503 HPV typing results, 10195 (780 positive, 9577 negative) were concordant, for an agreement of 97.1% (95% CI: 96.7-97.4) and an excellent of κ = 0.82 (95% CI: 0.80-0.84). The mean type-specific concordance for 27 genotypes was 97.0%, 95% CI: 95.8-98.5 (κ = 0.86 ± 0.07, 95% CI: 0.83-0.88). Excellent agreement was obtained individually for all high-risk genotypes (κ = 0.81-0.97) and for most other genotypes except for types 42, 44, 54, 68, and 69. The mean number of types per sample in discordant samples detected with LA-HPV (3.0, 95% CI: 2.7-3.4) was greater than in concordant samples (1.4, 95% CI: 1.3-1.5; p< 0.001). In conclusion, HPV-28 compared favorably with LA-HPV, but was more frequently positive for HPV42 and HPV68.