冷杉是松科的重要属,在亚洲的高地发现了大约50个物种,欧洲,北非,北美和中美洲。本工作的主要目的是研究冷杉的树脂和锥的化学含量和生物潜力。bornmulleriana和冷杉nordmanniana亚种。equi-trojani,分别。使用液相色谱-高分辨率质谱(LC-HRMS)评估树脂和视锥的类黄酮和酚类含量。此外,使用气相色谱-质谱(GC-MS)和气相色谱-火焰离子化检测器(GC-FID)分析精油和脂肪酸组成,分别。对某些癌细胞系筛选提取物和精油的细胞毒性,即,人前列腺腺癌细胞系(PC3),人肺腺癌细胞系(A549),人胰腺癌细胞系(PANC-1),人肝癌细胞系(HepG2),人乳腺癌细胞系(MDA-MB231),和正常人肺成纤维细胞系(CCD-34-LU),用MTT[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴化物]测定。根据MTT结果,正己烷萃取物(CH)和树脂萃取物(RH),乙醇-水(CEW),二氯甲烷(CD),锥体的丙酮(CA)提取物大多对HepG2细胞系造成细胞毒性。冷杉亚种的抗病毒活性。还在卵中评估了5μg/g和10μg/g剂量的提取物对禽类冠状病毒的杀病毒活性。冷杉nordmanniana亚种。提取物对无特定病原体的含胚鸡蛋表现出浓度依赖性的抗病毒活性。重要的是,锥形丙酮提取物(CA),浓缩乙醇提取物(CE),冷杉亚种和浓二氯甲烷提取物(CD)。在10μg/g的浓度下表现出对病毒的强烈抑制作用。用针叶树形式的乙醇-水提取物(CEW)观察到最有效的杀病毒活性。根据这些结果,事实证明,冷杉品种可能是潜在的,可持续,和可再生的药物来源,特别是考虑到令人印象深刻的抗病毒和显着的细胞毒活性潜力。
Abies is an important genus of the family Pinaceae, with about 50 species found in the highlands of Asia, Europe, North Africa, and North and Central America. The principal aim of the present work was to investigate the chemical content and biological potential of the resin and
cone from Abies nordmanniana subsp. bornmulleriana and Abies nordmanniana subsp. equi-trojani, respectively. The flavonoid and phenolic contents of the resin and cones were evaluated using liquid chromatography-high resolution mass spectrometry (LC-HRMS). Additionally, the essential oil and fatty acid compositions were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID), respectively. Cytotoxicity of the extracts and essential oils were screened against certain cancer cell lines, namely, human prostate adenocarcinoma cell line (PC3), human lung adenocarcinoma cell line (A549), human pancreatic cancer cell line (PANC-1), human hepatocellular carcinoma cell line (HepG2), human breast cancer cell line (MDA-MB231), and normal human lung fibroblast cell line (CCD-34-LU), with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay. According to the MTT results, hexane extracts of both
cone (CH) and resin (RH), ethanol-water (CEW), dichloromethane (CD), and acetone (CA) extracts of the
cone mostly inflict cytotoxicity in HepG2 cell line. Antiviral activities of Abies nordmanniana subsp. extracts at doses of 5 μg/g and 10 μg/g were also evaluated in ovo for their virucidal activity against avian coronavirus. Abies nordmanniana subsp. extracts exhibited concentration-dependent antiviral activity on specific pathogen-free embryonated chicken eggs. Significantly,
cone acetone extract (CA),
cone ethanol extract (CE), and
cone dichloromethane extract (CD) of Abies nordmanniana subsp. exhibited strong inhibition of the virus at a concentration of 10 μg/g. The most potent virucidal activity was observed with ethanol-water extract of conifer form (CEW). According to these results, it was proved that Abies nordmanniana species could be a potential, sustainable, and renewable drug source, especially considering the impressive antiviral and significant cytotoxic activity potentials.