目的:主要目的是开发一种伴随的等度超高效液相色谱光电二极管阵列检测方法,以评估Upadacitinib及其过程相关杂质:杂质-1和杂质-2。对应力环境下可能的降解物进行了进一步的验证和研究。
方法:使用的所有化学品和试剂均为HPLC(乙腈,甲醇)和分析级(三氟乙酸)。超高效液相色谱(Agilent1290InfinityIILC系统)由四元泵组成,BEHC18(50×2.1mm,1.7µ)列,和光电二极管阵列探测器。该方法是用乙腈:甲醇:0.1%v/v三氟乙酸(50:20:30v/v/v)流动相在0.2mL/min下开发的。在5.5分钟的运行时间内的流速。在231.2nm进行检测。
结果:分别达到的保留时间为2.289分钟。(Upadacitinib),0.972分钟(Upadacitinib杂质-1),和3.508分钟。(Upadacitinib杂质-2)。优化后的方法得到了进一步的验证,Upadacitinib和Upadacitinib杂质-1和2的线性范围分别在15.0µg/mL-180.0µg/mL和1.0-12.0µg/mL。检测和定量限为4.50µg/mL,15.00µg/mL(Upadacitinib)和0.30µg/mL,1.0µg/mL(Upadacitinib杂质1和2)。
结论:快速,isocratic,具体,根据ICHQ2(R1)指南研究,开发了可重复的超高效液相色谱方法,并对各种参数进行了验证。进行应力研究,将样品稀释物暴露于各种处理(酸,碱,过氧化物,HPLC水,热,和紫外线)。降解物与活性物质的峰分离良好。在降解期间观察到指示性质的稳定性。优化后的方法可用于药物领域片剂剂型中Upadacitinib及其过程相关杂质的分离和估算。
OBJECTIVE: The primary objective was to develop a
concomitant isocratic ultra-performance liquid chromatographic photo-diode array detection method to estimate Upadacitinib and its process-related impurities: impurity-1 and impurity-2. Further validation was conducted and studied for possible degradants under stress environments.
METHODS: All the chemicals and reagents used were of HPLC (acetonitrile, methanol) and analytical grade (trifluoro acetic acid). The ultra-performance liquid chromatography (Agilent 1290 Infinity II LC system) consists of a quaternary pump, a BEH C18 (50×2.1mm, 1.7μ) column, and photo-diode array detector. The method was developed with acetonitrile: methanol: 0.1% v/v trifluoro acetic acid (50:20:30 v/v/v) mobile phase at 0.2mL/min flow rate within a run time of 5.5min The detection was carried at 231.2nm.
RESULTS: The respective retention times achieved were 2.289min (Upadacitinib), 0.972min (Upadacitinib impurity-1), and 3.508min (Upadacitinib impurity-2). The optimized method was validated further, and the linearity range was best fit at 15.0-180.0μg/mL for Upadacitinib and 1.0-12.0μg/mL for both Upadacitinib impurity-1 and 2 respectively. The detection and quantification limits were 4.50μg/mL, 15.00μg/mL (Upadacitinib) and 0.30μg/mL, 1.0μg/mL (Upadacitinib impurity-1 and 2).
CONCLUSIONS: A fast, isocratic, specific, and reproducible ultra-performance liquid chromatographic method was developed and validated for various parameters according to the ICH Q2 (R1) guidelines studies. Stress studies were conducted exposing the sample dilution to various treatments (acid, alkali, peroxide, HPLC water, heat, and UV light). The degradants were well-separated apart from the peaks of the active substance. The stability indicating nature was observed during the degradation. The optimized method can be applied for the separation and estimation of Upadacitinib and its process-related impurities in pharma sector in tablet dosage forms.