BACKGROUND: Penicillium oxalicum is an important fungal agent in the composting of cattle manure, but the changes that occur in the microbial community, physicochemical factors, and potential functions of microorganisms at different time points are still unclear. To this end, the dynamic changes occurring in the microbial community and physicochemical factors and their correlations during the composting of cattle manure with Penicillium oxalicum were analysed.
RESULTS: The results showed that the main phyla observed throughout the study period were Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes, Halanaerobiaeota, Apicomplexa and Ascomycota. Linear discriminant analysis effect size (LEfSe) illustrated that Chitinophagales and Eurotiomycetes were biomarker species of bacteria and eukaryote in samples from Days 40 and 35, respectively. Bacterial community composition was significantly correlated with temperature and pH, and eukaryotic microorganism community composition was significantly correlated with moisture content and NH4+-N according to redundancy analysis (RDA). The diversity of the microbial communities changed significantly, especially that of the main pathogenic microorganisms, which showed a decreasing trend or even disappeared after composting.
CONCLUSIONS: In conclusion, a combination of high-throughput sequencing and physicochemical analysis was used to identify the drivers of microbial community succession and the composition of functional microbiota during cattle manure composting with Penicillium oxalicum. The results offer a theoretical framework for explaining microecological assembly during cattle manure composting with Penicillium oxalicum.

The present study aimed to i) assess the disintegration of a novel bio-packaging during aerobic composting (2 and 6 % tested concentrations) and evaluate the resulting compost ii) analyse the ecotoxicity of bioplastics residues on earthworms; iii) study the microbial communities during composting and in \'earthworms\' gut after their exposure to bioplastic residues; iv) correlate gut microbiota with ecotoxicity analyses; v) evaluate the chemico-physical characterisation of bio-packaging after composting and earthworms\' exposure. Both tested concentrations showed disintegration of bio-packaging close to 90 % from the first sampling time, and compost chemical analyses identified its maturity and stability at the end of the process. Ecotoxicological assessments were then conducted on Eisenia fetida regarding fertility, growth, genotoxic damage, and impacts on the gut microbiome. The bioplastic residues did not influence the earthworms\' fertility, but DNA damages were measured at the highest bioplastic dose tested. Furthermore bioplastic residues did not significantly affect the bacterial community during composting, but compost treated with 2 % bio-packaging exhibited greater variability in the fungal communities, including Mortierella, Mucor, and Alternaria genera, which can use bioplastics as a carbon source. Moreover, bioplastic residues influenced gut bacterial communities, with Paenibacillus, Bacillus, Rhizobium, Legionella, and Saccharimonadales genera being particularly abundant at 2 % bioplastic concentration. Higher concentrations affected microbial composition by favouring different genera such as Pseudomonas, Ureibacillus, and Streptococcus. For fungal communities, Pestalotiopsis sp. was found predominantly in earthworms exposed to 2 % bioplastic residues and is potentially linked to its role as a microplastics degrader. After composting, Attenuated Total Reflection analysis on bioplastic residues displayed evidence of ageing with the formation of hydroxyl groups and amidic groups after earthworm exposure.

The system of fertilizing agricultural soils with sludge or compost from wastewater treatment processes, as one of the principles of the circular economy, can lead to microplastic (MP) contamination. The existing technical standards for fertilization are very recent and do not consider this problem, although there is scientific evidence of their existence. Therefore, this study, on the one hand, evaluates the presence of MPs in agricultural soils, previously treated with sludge or compost from wastewater treatment plants for fertilization, and on the second hand, it studies the effect of these MPs on earthworms in three different locations in the south of Spain. For the study, selected composts deriving from the different stages of the composting process and three fertilized soils with increasing MP doses were followed. Samples were taken from different sections in depth (0-5, 5-10, and 10-20 cm) to study the shape, size, type, and abundance of MPs using infrared spectroscopy (FTIR). The results showed that the most abundant shape was fiber, followed by fragment and finally bulk, for both composts and soils. Regarding size distribution, 100 µm was the predominant size in composts (64.3% ± 9.8), while in the case of soils, the predominant range was from 100 to 500 µm. The prevalent polymers in both, composts and soils, were PTFE, TPE, PP, and PET, with four times higher amounts in composts than in soils. Ingestion of common MPs were also verified in two earthworm species, which ingested concentrations higher than 2.1% w/w. PP was the most ingested MP and Eisenia fetida was more voracious compared with Lumbricus terrestris. Therefore, it can be considered a suitable bioindicator for monitoring microplastic contamination in agricultural soil.

Restoring native plant species on degraded landscapes is challenging. Symbiotic partners in the plant rhizosphere can aid in nutrient acquisition, pathogen protection, stress tolerance, and many other processes. However, these microbes are often absent in altered landscapes and need to be re-integrated to improve restoration efforts. We evaluated, within a laboratory setting, the ability of commercial and indigenous rhizobia strains to form nodules on lupine species used for rangeland seedings in the Great Basin region of the Western United States and ascertained if these strains could be applied through a seed coating. We also evaluated if a compost amendment applied via seed coating could further enhance the performance of the rhizobia strains. Our analysis showed that successful nodulation could occur using commercial and wildland-collected indigenous strains through either a liquid culture applied to seedlings or as a dry seed coating. However, the number of root nodules and the presence of a pink color (indicating nitrogen fixation) were typically higher in the commercial product than in the indigenous strains. Compost did not improve nodulation or the performance of the nodules; however, this treatment alone improved shoot growth. Overall, these results suggest that commercial rhizobium may be more effective in improving plant growth, and future research with native rhizobia may want to consider identifying strains compatible with seed-coating delivery. Longer-term studies are now merited for assessing how the rhizobia strains evaluated in this study influence plant growth, particularly in a field setting.

Argania spinosa (L.) Skeels is an endemic species to Morocco that has multiple uses. It plays multiple important roles in terms of its botanical, ecological, and economic properties. However, the domestication of this species will open up considerable economic opportunities for Morocco. Here, for the first time, we assessed the effect of different doses of compost and NPK fertilizers on the vegetative growth parameters, biochemical and antioxidant potential of the Argania spinosa plant, and soil properties. Over a two-year period (2022-2023), eight different treatments were applied across two experimental sites. These treatments included the following: T0 (Control), T1 (F1-80.50.70 g NPK/plant), T2 (F1-125.75.100 g NPK/plant), T3 (F2-160.100.140 g NPK/plant), T4 (F2-250.150.200 g NPK/plant), T5 (F1-2.5 kg/plant compost), T6 (F1-5 kg/plant compost), T7 (F2-5 kg/plant compost), and T8 (F2-10 kg/plant compost), with F1 and F2 being the frequencies of application. We compared several doses of fertilizers with no fertilization as a control. The results showed a significant influence of the compost and NPK fertilizer on the vegetative growth parameters. For the Tamjlojt site, the first year is important because treatments T3 and T4 significantly increased height by 71.94 ± 21.15% and 74.31 ± 12.31%, respectively. For the circumference, the results showed a significant improvement by the treatments T4 and T3, and T1 demonstrated the highest gain. For the collar diameter, all treatments showed a significant difference. The most notable difference was observed with treatments T3 and T7 with 115.63 ± 33.88% and 101.09 ± 20.84%, respectively. For the Rasmouka site, the second year was the most important. The treatments with the most important height increase were T7 and T8, with a value of 43.14 ± 10.06% and 36.44 ± 9.95%; the same was observed for collar diameter as a significant increase was found in T8 and T7 with a value of 55.05 ± 15.7% and 54.08 ± 9.64%. For the circumference parameter, the treatments that increased significantly this parameter were T8 and T7 with a value of 53.36 ± 15.11% and 50.34 ± 11.29% in 2023. In addition, the highest content of carbohydrates was recorded for the treatment T3 with a value of 148.89 ± 8.11 (mg EG/g). For phenolic determination, the highest value was 2532 ± 457.13 (µg GAE/mL), shown for treatment T1. For flavonoids, the treatments that showed a significant effect were T1 and T6 with a value of 2261.98 ± 184.61 and 1237.70 ± 95.65 (µg QE/mL), respectively. For the impact on soil properties, the electrical conductivity, at the Tamjlojt site, treatment T1 showed a significant increase to 1139.00 ± 241.30 (ms/cm), while at the Rasmouka site, treatment T8 showed a significant increase to 303.33 ± 9.33 (ms/cm). Concerning organic carbon, all treatments resulted in increased percentages of this parameter in the soil. For the Tamjlojt site, the T7 treatment had a significant positive effect on this parameter with a value of 0.87 ± 0.12%. For the Rasmouka site, the T3 treatment increased the percentage of organic carbon with a value of 1.17 ± 0.07%. In addition, the organic matter content showed an improvement with a value of 2.02 ± 0.12%. As there are no previous studies in Argania spinosa fertilization, this study greatly contributes to our understanding of the benefits of using different fertilizers at different doses, in particular T8 and T7 as organic fertilizers and T3, T4 as chemical ones, on argan growth, the biochemical and antioxidant properties of leaves, and its soil properties.

With growing awareness of the environmental, economic, and social costs associated with food waste, there is a concerted effort on multiple scales to recover the nutrient value of discarded food. These developments are positive, but the rapid movement toward alternatives and the complexity of solving problems located at the intersection of economic, social, and environmental systems also have the potential to produce unanticipated risks. This paper draws upon long-term stakeholder-engaged research throughout New England, with a focus on Maine, to develop a transdisciplinary, systems-based model of the potential social, economic, and environmental risks of food waste nutrient cycling. Our effort is intended to help inform the creation of safe, functional, and environmentally benign circular food systems.

Dry bean (Phaseolus vulgaris L.) is an important commercialized field crop in South Africa for aiding in food security as a cheap protein source. However, it is highly susceptible to root-knot nematodes (RKN), Meloidogyne species. Use of indigenous nematophagous fungi as bio-control agents (BCA) of Meloidogyne nematodes is a promising research focus area. This is because indigenous fungal species are naturally part of the ecosystem and therefore compatible with other biological processes unlike most synthetic chemicals. The objective of the study was to identify indigenous nematophagous fungal BCA and establish their potential efficacy in reducing M. enterolobii population densities on dry bean with and without incorporation of compost. Screened indigenous fungal species included Aspergillus terreus, Talaromyces minioluteus, T. sayulitensis, Trichoderma ghanense, and T. viride. There were observed significant parasitism differences (P ≤ 0.05) among the BCA, with T. ghanense showing the highest egg parasitism (86%), followed by T. minioluteus (72%) and T. sayulitensis (70%). On the other hand, the highest J2 parasitism was observed on T. minioluteus (95%), followed by A. terreus and T. viride (63%). A similar trend was observed under in vivo conditions, with higher efficacy with compost incorporation. This provides a highly encouraging alternative and ecologically complementary Meloidogyne management in dry bean production.

The rising utilization of PLA/PBAT-ST20 presents potential ecological risks stemming from its casual disposal and incomplete degradation. To solve this problem, this study investigated the degradation capabilities of PLA/PBAT-ST20 by a co-culture system comprising two thermophilic bacteria, Pseudomonas G1 and Kocuria G2, selected and identified from the thermophilic phase of compost. Structural characterization results revealed that the strains colonized the PLA/PBAT-ST20\'s surface, causing holes and cracks, with an increase in the carbonyl index (CI) and polydispersity index (PDI), indicating oxidative degradation. Enzyme activity results demonstrated that the co-culture system significantly enhanced the secretion and activity of proteases and lipases, promoting the breakdown of ester bonds. LC-QTOF-MS results showed that various intermediate products were obtained after degradation, ultimately participating in the TCA cycle (ko00020), further completely mineralized. Additionally, after 15-day compost, the co-culture system achieved a degradation rate of 72.14 ± 2.1 wt% for PBAT/PLA-ST20 films, with a decrease in the abundance of plastic fragments of all sizes, demonstrating efficient degradation of PLA/PBAT-ST20 films. This study highlights the potential of thermophilic bacteria to address plastic pollution through biodegradation and emphasizes that the co-culture system could serve as an ideal solution for the remediation of PLA/PBAT plastics.

Organic wastes (OWs) can be a common source of copper (Cu) contamination of agricultural soils. Here we conducted a comprehensive study of 22 raw and treated OWs sampled at 6 different full-scale OW treatment plants. Bulk XANES analysis findings indicated that the Cu oxidation state was subject to changes throughout the OW treatment process, mostly depending on the anaerobic/aerobic conditions prevailing in each treatment stage. These changes were independent of the OW origin (agricultural, urban or industrial). Cu(I) prevailed in raw OWs and digestates (88-100%), whereas Cu(II) dominated in composts (46-100%). Bulk EXAFS analysis confirmed these observations and revealed that Cu(I) species in raw OWs and digestates consisted mainly of Cu(I)-sulfide (76-100%), while Cu(II) species (60-100%) in composts were Cu(II)-citrate, Cu(II)-carbonate and amorphous Cu(II)-phosphate. Interestingly, we observed that anaerobic digestion was conducive to the formation of crystallized Cu(I)-sulfides at the expense of nanosized and poorly crystalline Cu(I)-sulfide species, and that the recalcitrant Cu(I) species in composts was always crystallized Cu(I)-sulfide. XANES imaging analysis revealed Cu(II) species present in low proportions (2-4%) that were not detected using bulk XAS analysis in raw OWs and digestates. This demonstrated the potential of XANES imaging for probing minor species in complex matrices.

Farmers are using municipal wastewater either treated or untreated for irrigation because of limited fresh water resources. Wastewater extensively used for irrigation purposes is enriched with many nutrients. The reuse of wastewater is imposing a negative impact on human health and the ecosystem. It is a need of the day to identify and assess issues of the reuse of wastewater. In the current experiment, impact of organic/inorganic amendments was studied to mitigate the toxic effects of pollutants present in wastewater. Soil was brought from the site having consistent use of wastewater and different treatments were applied as per plan. The experiment has 28 treatments with 04 replications. Nine different amendments were used at 3 varying levels. Incubation time of 30 days was given after the addition of all treatments. The results of the study showed the application of FYM @ 5.0% w/w soil reduced soil pH (7.44), EC (2.16 dS m-1), SAR (8.14), lead (8.48 mg kg-1), cadmium (1.14 mg kg-1), nickel (10.55 mg kg-1) and arsenic (2.03 mg kg-1) when compared with control and other treatments. Usage of compost and horse waste followed FYM. On the basis of this study, it is recommended that wastewater can be used for irrigation purpose after treating with FYM preferably and compost in general.