在这项研究中,使用各种参数,例如发芽率,研究了荨麻提取物(Udex)对洋葱中Li2CO3毒性的保护作用,根系生长,体重增加,有丝分裂指数(MI),丙二醛(MDA),微核(MN),抗氧化酶活性,染色体异常(CAs)和解剖学变化。由于Udex的生物活性与其活性含量有关,通过LC-MS/MS分析确定酚类化合物的概况。Li2CO3导致测试参数异常和发芽参数严重退化。施用100mg/LLi2CO3可使叶绿素a和b含量降低73.04%和65.7%,分别。Li2CO3的应用通过诱导MI的显着降低和MN的频率增加而表现出细胞毒性作用。并通过引起CA表现出遗传毒性作用。100mg/LLi2CO3处理后,MDA,脯氨酸,超氧化物歧化酶,过氧化氢酶水平增加了54.9%,58.5%,47.8%,52.3%,分别。Li2CO3和Udex共同给药导致生化参数和遗传毒性参数增加的回归,和发芽参数的改善。此外,Udex在减轻Li2CO3对根尖的有害影响方面表现出功效,特别是在200µg/mLUdex治疗组中。皮质细胞壁和传导组织的增厚,这通常是由Li2CO诱导的,在Udex治疗组中未观察到。Udex的保护作用可以通过其含有的酚类化合物来解释。在Udex中检测到芦丁是主要成分,其他酚类按其存在率列出为保护茶酸>咖啡酸>对香豆酸>丁香酸>迷迭香酸>表儿茶素。锂离子,工业化后环境的增加,是一种重要的环境污染物,并表现出影响生物体许多途径的毒性。科学研究不仅应该检测这些毒性作用,而且应该开发解决这些问题的方法。在这项研究中,确定Udex应用对Li2CO3毒性具有毒性降低作用。此外,已经证明A.cepa是确定这种毒性和降低毒性应用的重要指标。
In this study, the protective role of Urtica dioica extract (Udex) against Li2CO3 toxicity in Allium cepa L. was investigated using various parameters such as germination rates, root growth, weight gain, mitotic index (MI), malondialdehyde (MDA), micronucleus (MN), antioxidant enzyme activity, chromosomal abnormalities (CAs) and anatomical changes. As the biological activity of Udex is related to its active content, the profile of phenolic compounds was determined by LC-MS/MS analysis. Li2CO3 caused abnormalities in the tested parameters and serious regressions in germination parameters. Application of 100 mg/L Li2CO3 reduced the chlorophyll a and b contents by 73.04% and 65.7%, respectively. Li2CO3 application exhibited a cytotoxic effect by inducing significant decreases in MI and increases in the frequency of MN, and also showed a genotoxic effect by causing CAs. After 100 mg/L Li2CO3 treatment, MDA, proline, superoxide dismutase, and catalase levels increased by 54.9%, 58.5%, 47.8%, and 52.3%, respectively. Li2CO3 and Udex co-administration resulted in a regression in increased biochemical parameters and genotoxicity parameters, and an improvement in germination parameters. Furthermore, Udex demonstrated efficacy in mitigating the detrimental effects of Li2CO3 on the root tip, particularly in the 200 µg/mL Udex-treated group. The thickening of the cortex cell wall and conduction tissue, which is commonly induced by Li₂CO₃, was not observed in the Udex-treated group. The protective effect of Udex can be explained by the phenolic compounds it contains. Rutin was detected as the major component in Udex and other phenolics were listed according to their presence rate as protecatechuic acid > caffeic acid > p-coumaric acid > syringic acid > rosemarinic acid > epicatechin. Li ions, which increase in the environment after industrialization, are an important environmental pollutant and exhibit toxicity that affects many pathways in organisms. Scientific research should not only detect these toxic effects but also develop solutions to such problems. In this study, it was determined that the Udex application had a toxicity-reducing role against Li2CO3 toxicity. Also, it has been demonstrated that A. cepa is an important indicator in determining this toxicity and toxicity-reducing applications.