生物硫醇分析为健康评估提供了重要的见解，并有助于早期发现潜在的健康问题。从而能够进行及时有效的干预。在这项研究中,我们开发了具有多种酶活性的超小CuMn-组氨酸（His）纳米酶。通过氢键和静电作用实现了在中性pH下CuMn-His增强的过氧化物酶（POD）样活性。此外,CuMn-His在中性pH下具有漆酶（LAC）样和超氧化物歧化酶（SOD）样活性。基于CuMn-His在中性pH下三种不同的酶模拟活性，成功构建了无需更换缓冲溶液的比色传感阵列。该阵列已成功用于鉴定三种生物硫醇，谷胱甘肽(GSH),半胱氨酸(Cys),同型半胱氨酸（Hcy）。随后，在复杂的血清和细胞水平分析中显示了出色的应用结果。本研究为开发具有多种酶活性的超小双金属纳米酶和构建比色传感阵列提供了创新策略。
Biothiol assays offer vital insights into health assessment and facilitate the early detection of potential health issues, thereby enabling timely and effective interventions. In this study, we developed ultrasmall CuMn-Histidine (His) nanozymes with multiple enzymatic activities. CuMn-His enhanced peroxidase (POD)-like activity at neutral pH was achieved through hydrogen bonding and electrostatic effects. In addition, CuMn-His possesses laccase (LAC)-like and superoxide dismutase (SOD)-like activities at neutral pH. Based on three different enzyme mimetic activities of CuMn-His at neutral pH, the colorimetric sensing array without changing the buffer solution was successfully constructed. The array was successfully used for the identification of three biothiols, glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). Subsequently, excellent application results were shown in complex serum and cellular level analyses. This study provides an innovative strategy for the development of ultrasmall bimetallic nanozymes with multiple enzymatic activities and the construction of colorimetric sensing arrays.
