背景:新的证据将肠道微生物群与各种人类疾病联系起来,包括结直肠癌(CRC)的发生和发展。然而,与CRC复发和患者预后相关的肠道菌群分布尚未完全了解,尤其是在中国队列中。
目的:探讨肠黏膜菌群分布与CRC复发及患者预后的关系。
方法:我们获得了从诊断为CRC的75例患者和26例健康对照中收集的肠道微生物群的组成和结构。对患者进行常规随访以确定肿瘤是否复发。来自肿瘤上的三联体配对样本,我们通过16S核糖体RNA测序,对诊断有/无CRC复发患者的邻近肿瘤部位和肿瘤外部位进行了分析,以评估肠道粘膜菌群的空间特异性模式.接下来,我们进行了生物信息学分析,Kaplan-Meier生存分析和Cox回归分析以确定肠粘膜微生物群分布与CRC复发和患者预后之间的关系。
结果:我们观察到与CRC复发和患者预后相关的肠道粘膜菌群的空间特异性模式。共有17个细菌属/家族被鉴定为CRC复发和患者预后的潜在生物标志物。包括anaerotruncus,拟杆菌,科氏杆菌科,Dialister,真细菌,梭杆菌,Filifactor,Gemella,嗜血杆菌,莫吉杆菌,吡喃杆菌,Parvimonas,卟啉科,Slackia,Schwartzia,TG5和密螺旋体。
结论:我们的工作表明,肠道菌群可以作为预测CRC复发和患者死亡风险的生物标志物。
BACKGROUND: Emerging evidence links gut microbiota to various human diseases including colorectal cancer (CRC) initiation and development. However, gut microbiota profiles associated with CRC recurrence and patient prognosis are not completely understood yet, especially in a Chinese cohort.
OBJECTIVE: To investigate the relationship between gut mucosal microbiota profiles and CRC recurrence and patient prognosis.
METHODS: We obtained the composition and structure of gut microbiota collected from 75 patients diagnosed with CRC and 26 healthy controls. The patients were followed up by regular examination to determine whether tumors recurred. Triplet-paired samples from on-tumor, adjacent-tumor and off-tumor sites of patients diagnosed with/without CRC recurrence were analyzed to assess spatial-specific patterns of gut mucosal microbiota by 16S ribosomal RNA sequencing. Next, we carried out bioinformatic analyses, Kaplan-Meier survival analyses and Cox regression analyses to determine the relationship between gut mucosal microbiota profiles and CRC recurrence and patient prognosis.
RESULTS: We observed spatial-specific patterns of gut mucosal microbiota profiles linked to CRC recurrence and patient prognosis. A total of 17 bacterial genera/families were identified as potential biomarkers for CRC recurrence and patient prognosis, including Anaerotruncus, Bacteroidales, Coriobacteriaceae, Dialister, Eubacterium, Fusobacterium, Filifactor, Gemella, Haemophilus, Mogibacteriazeae, Pyramidobacter, Parvimonas, Porphyromonadaceae, Slackia, Schwartzia, TG5 and Treponema.
CONCLUSIONS: Our work suggests that intestinal microbiota can serve as biomarkers to predict the risk of CRC recurrence and patient death.