背景:黄土汤(HTD),一种传统的中药配方,温暖脾脏,滋养血液,止血.它已经被用来治疗痢疾,消化道出血,腹泻,以及在中国两千多年来由脾阳虚引起的其他症状。然而,使用HTD治疗脾阳虚(CDSD)引起的慢性腹泻的潜在机制尚不清楚。
目的:本研究使用小鼠模型研究HTD是否能介导肠道菌群和血清代谢产物改善CDSD症状。
方法:构建番泻叶和异常饮食诱导的CDSD小鼠模型。通过测量其体重来评估HTD在12.5、25.0和50.0g/kg/d时对CDSD小鼠的调节作用,腹泻率,稀便率,和组织病理学。通过16SrRNA基因测序分析CDSD小鼠肠道菌群的变化。使用超高效液相色谱-质谱/质谱(UHPLC-MS/MS)进行非靶向血清代谢组学分析。
结果:HTD通过减少体重减轻对CDSD有调节作用,腹泻率,稀便率,和病理损害。肠道菌群分析表明,HTD通过降低Allobaculum的丰度改变了群落组成,乳酸菌,和Ruminococus.血清代谢组学显示抗坏血酸和醛盐代谢,醛固酮的合成和分泌,血小板活化,缺氧诱导因子1信号通路,肌醇磷酸代谢,磷脂酰肌醇信号,半乳糖代谢,HTD处理后调节α-亚麻酸代谢。
结论:HTD可以通过减少体重减轻来缓解CDSD症状,腹泻率,稀便率,以及CDSD小鼠肠道菌群和血清代谢产物的建模和调节引起的病理损伤。
BACKGROUND: Huangtu decoction (HTD), a traditional Chinese medicine recipe, warms the spleen, nourishes the blood, and stops bleeding. It has been used to treat dysentery, gastrointestinal bleeding, diarrhea, and other symptoms caused by spleen-yang deficiency for more than 2,000 years in China. However, the mechanism underlying the treatment of chronic diarrhea due to spleen-yang deficiency (CDSD) using HTD remains unclear.
OBJECTIVE: This study investigated whether HTD could mediate intestinal flora and serum metabolites to improve CDSD symptoms using a mouse model.
METHODS: A CDSD mouse model induced by senna and an abnormal diet was constructed. The regulatory effects of HTD at 12.5, 25.0, and 50.0 g/kg/d on CDSD mice were assessed by measuring their bodyweight, diarrhea rate, loose stool rate, and histopathology. Changes in the intestinal flora of CDSD mice were analyzed by 16S rRNA gene sequencing. Untargeted serum metabolomic analysis was performed using ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UHPLC-MS/MS).
RESULTS: HTD had a modulating effect on CDSD by reducing the weight loss, diarrhea rate, loose stool rate, and pathologic damage. Intestinal flora analysis showed that HTD altered the community composition by decreasing the abundance of Allobaculum, Lactobacillus, and Ruminococcus. Serum metabolomics revealed that ascorbate and aldarate metabolism, aldosterone synthesis and secretion, platelet activation, hypoxia-inducible factor 1 signaling pathway, inositol phosphate metabolism, phosphatidylinositol signaling, galactose metabolism, and alpha-linolenic acid metabolism were modulated after HTD treatment.
CONCLUSIONS: HTD may alleviate CDSD symptoms by reducing weight loss, diarrhea rate, loose stool rate, and pathologic damage caused by modeling and regulating intestinal flora and serum metabolites in CDSD mice.