在这里,我们提供了紫色黄油蛤仔(Saxidomuspurpuratus)的第一个全基因组序列,一种经济上重要的双壳贝类。具体来说,我们对Sa的基因组进行了测序和从头组装。基于Promethion长读数和Hi-C数据的紫癜。Sa的978-Mb基因组。紫癜包含19条染色体,具有36,591个预测的蛋白质编码基因。Sa的N50长度。紫癜的基因组是52Mb,显示双壳类动物基因组中最高的连续组装。通用单拷贝直系同源物评估的基准标记表明,在装配中存在95.07%的完整后生通用单拷贝直系同源物(n=954)。大约51%的Sa。紫癜的基因组包含重复序列。基于高品质的Sa。紫癜的基因组,我们解析了一半的免疫相关基因,即,清道夫受体(SR)蛋白,它们与密切相关的青蟹基因组中的那些共线。这一发现表明免疫相关基因之间的高度保守性。22(19%)SR蛋白在Sa中串联重复。紫癜的基因组,暗示了推定的趋同进化。总的来说,Sa.紫癜的基因组为发现经济上重要的性状和免疫反应基因提供了新的资源。
Herein, we provide the first whole-genome sequence of the purple butter clam (Saxidomus purpuratus), an economically important bivalve shellfish. Specifically, we sequenced and de novo assembled the genome of Sa. purpuratus based on PromethION long reads and Hi-C data. The 978-Mb genome of Sa. purpuratus comprises 19 chromosomes with 36,591 predicted protein-coding genes. The N50 length of Sa. purpuratus genome is 52 Mb, showing the highest continuous assembly among bivalve genomes. The Benchmarking by Universal Single-Copy Orthologs assessment indicated that 95.07% of complete metazoan universal single-copy orthologs (n = 954) were present in the assembly. Approximately 51% of Sa. purpuratus genome comprises repetitive sequences. Based on the high-quality Sa. purpuratus genome, we resolved half of the immune-associated genes, namely, scavenger receptor (SR) proteins, which are collinear to those in the closely related Cyclina sinensis genome. This finding suggested a high degree of conservation among immune-associated genes. Twenty-two (19%) SR proteins are tandemly duplicated in Sa. purpuratus genome, suggesting putative convergence evolution. Overall, Sa. purpuratus genome provides a new resource for the discovery of economically important traits and immune-response genes.