本研究旨在探讨黄曲霉毒素B1(AFB1)对鸭肝胆汁淤积的影响及其营养调控作用。将三百六十只1日龄鸭子随机分为六组,喂养4周。对照组饲喂基本日粮,而实验组饮食中含有90μg/kg的AFB1。胆甾胺,阿托伐他汀钙,牛磺酸,和大黄素添加到四个实验组的饮食中。结果表明,在AFB1组中,生长特性,总胆汁酸(TBA)血清水平和总超氧化物歧化酶(T-SOD),谷胱甘肽过氧化物酶(GSH-Px),和谷胱甘肽(GSH)肝脏水平下降,而肝脏丙二醛(MDA)和TBA水平升高(p<0.05)。此外,AFB1引起胆汁淤积。胆甾胺,阿托伐他汀钙,牛磺酸,大黄素可以降低血清和肝脏的TBA水平(p<0.05),缓解胆汁淤积的症状。qPCR结果显示,AFB1上调肝脏中细胞色素P450家族7亚家族A成员1(CYP7A1)和细胞色素P450家族8亚家族B成员1(CYP8B1)基因表达,下调ATP结合盒亚家族B成员11(BSEP)基因表达,牛磺酸和大黄素下调CYP7A1和CYP8B1基因表达(p<0.05)。总之,AFB1对健康产生负面影响,并改变与肝脏胆汁酸代谢相关的基因的表达,导致胆汁淤积。胆甾胺,阿托伐他汀钙,牛磺酸,大黄素可减轻AFB1诱导的胆汁淤积。
The aim of this study was to investigate the effects of aflatoxin B1 (AFB1) on cholestasis in duck liver and its nutritional regulation. Three hundred sixty 1-day-old ducks were randomly divided into six groups and fed for 4 weeks. The control group was fed a basic diet, while the experimental group diet contained 90 μg/kg of AFB1.
Cholestyramine, atorvastatin calcium, taurine, and emodin were added to the diets of four experimental groups. The results show that in the AFB1 group, the growth properties, total bile acid (TBA) serum levels and total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and glutathione (GSH) liver levels decreased, while the malondialdehyde (MDA) and TBA liver levels increased (p < 0.05). Moreover, AFB1 caused cholestasis.
Cholestyramine, atorvastatin calcium, taurine, and emodin could reduce the TBA serum and liver levels (p < 0.05), alleviating the symptoms of cholestasis. The qPCR results show that AFB1 upregulated cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and cytochrome P450 family 8 subfamily B member 1 (CYP8B1) gene expression and downregulated ATP binding cassette subfamily B member 11 (BSEP) gene expression in the liver, and taurine and emodin downregulated CYP7A1 and CYP8B1 gene expression (p < 0.05). In summary, AFB1 negatively affects health and alters the expression of genes related to liver bile acid metabolism, leading to cholestasis.
Cholestyramine, atorvastatin calcium, taurine, and emodin can alleviate AFB1-induced cholestasis.