冷杉属广泛分布于世界各地,对林业具有重要意义。由于叶绿体通常是单亲遗传的,它们是解决基因流动等特定科学问题的重要工具,亲子关系,迁移和,总的来说,进化分析。在针叶树中建立的细胞器遗传标记相当限于RFLP标记,更多的劳动力和时间密集,与SSR标记相比。使用QUIAGENCLCWorkbench23.03,我们将来自不同冷杉物种的两个叶绿体基因组(NCBI种质:NC_039581,NC_042778,NC_039582,NC_042410,NC_035067,NC_062889,NC_042775,NC_057314,NC_041464,MH706244,MH047653潜在使用NCBI引物Blast服务器应用程序进行正向和反向引物的进一步选择和开发。在这篇文章中,我们为各种冷杉物种引入了一个非常多态的SSR标记集,这对其他针叶树属可能有用,比如Cedrus,松树,Pseudotsuga或Picea。总的来说,17个cpSSRs显示出可靠的扩增和多态性,总共检测到68个单倍型。在测试的冷杉属中扩增了所有17个cpSSR。在其他测试物种中,除了Taxusbaccata,至少一个引物是多态的。
The genus Abies is widely distributed across the world and is of high importance for forestry. Since chloroplasts are usually uniparentally inherited, they are an important tool for specific scientific issues like gene flow, parentage, migration and, in general, evolutionary analysis. Established genetic markers for organelles in conifers are rather limited to RFLP markers, which are more labour and time intensive, compared with SSR markers. Using QUIAGEN CLC Workbench 23.03, we aligned two
chloroplast genomes from different Abies species (NCBI accessions: NC_039581, NC_042778, NC_039582, NC_042410, NC_035067, NC_062889, NC_042775, NC_057314, NC_041464, MH706706, MH047653 and MH510244) to identify potential SSR candidates. Further selection and development of forward and reverse primers was performed using the NCBI Primer Blast Server application. In this article, we introduce a remarkably polymorphic SSR marker set for various Abies species, which can be useful for other conifer genera, such as Cedrus, Pinus, Pseudotsuga or Picea. In total, 17 cpSSRs showed reliable amplification and polymorphisms in A. grandis with a total of 68 haplotypes detected. All 17 cpSSRs amplified in the tested Abies spp. In the other tested species, except for Taxus baccata, at least one primer was polymorphic.