该研究旨在通过负载在广泛可用的天然聚合物(壳聚糖,C).因此,制备了负载SAE的壳聚糖纳米颗粒(CsNPs)。该发现说明了通过透射电子显微镜(TEM)证明的SAE负载的CsNP的球形颗粒的形成。此外,CsNP和SAE负载CsNP的平均粒径为131.8±24.63和318.5±73.94nm,分别。扫描电子显微镜(SEM)显示,由于SAE在表面上沉积并封装在CsNP的孔中,因此在CsNP的表面上沉积了许多球形颗粒。它还显示了钠等元素的存在,钾,铜,镁,锌,钙,铁,以及伴随CsNP的元素:碳,氧气,氮,和磷。提取物富含生物活性成分,如茴香脑,莽草酸,和不同的类黄酮,有助于其药用品质。通过色谱分析评估SAE中的生物活性分子。使用琼脂孔扩散测试,评估了CsNPs和SAE负载的CsNPs对与肺部疾病相关的病原菌的抗菌质量。最显著的抑制区显示负载SAE的CsNP具有最大的抗菌活性。使用MTT测定的抗癌活性用于生物学评估以确定针对NCl-H460肺癌细胞系的细胞毒性。结果表明,负载SAE的CsNPs以剂量依赖的方式显著降低细胞活力,SAE负载的CsNP具有最显著的抗癌影响。此外,肺癌治疗的体内试验表明,与其他治疗组相比,SAE加载的CsNPs组显示出肿瘤生物标志物和炎症的最大减少,如血浆丙二醛(MDA)水平降低,肿瘤蛋白53(p53),肿瘤坏死因子-α(TNF-α),和纤连蛋白.结果得出结论,这些彻底的表征,生物学评估,和抗菌测试已经证实SAE有效地整合到CsNP中。Further,SAE负载的CsNP可能是解决肺癌和细菌感染灭活的各种生物医学应用的合适选择。
The research study aimed to maximize the important medical role of star anise extract (SAE) through its loading on a widely available natural polymer (chitosan, Cs). Thus, SAE loaded chitosan nanoparticles (CsNPs) was prepared. The finding illustrated the formation of spherical particles of SAE loaded CsNPs as proved by transmission electron microscope (TEM). In addition, the average particle size of CsNPs and SAE loaded CsNPs are 131.8 ± 24.63 and 318.5 ± 73.94 nm, respectively. Scanning electron microscope (SEM) showed the presence of many spherical particles deposited on the surface of CsNPs owing to the deposition of SAE on the surface and encapsulated into pores of CsNPs. It also showed the presence of elements such as sodium, potassium, copper, magnesium, zinc, calcium, and iron, as well as the elements that accompanied with CsNPs: carbon, oxygen, nitrogen, and phosphorus. The extract was rich in bioactive components, such as anethole, shikimic acid, and different flavonoids, contributing to its medicinal qualities. The bioactive molecules in SAE were assessed by chromatographic analysis. Using the agar well diffusion test, the antibacterial qualities of CsNPs and SAE loaded CsNPs were evaluated against pathogenic bacteria linked to lung illnesses. The most significant inhibition zones showed that the SAE loaded CsNPs had the most antibacterial activity. The anticancer activity using MTT assay was used in the biological assessments to determine the cytotoxicity against the NCl-H460 lung cancer cell line. The results showed that CsNPs loaded with SAE considerably decreased cell viability in a dose-dependent manner, with the most significant anticancer impact by SAE loaded CsNPs. Furthermore, in vivo tests on lung cancer therapy revealed that when compared to other treatment groups, the SAE loaded CsNPs group showed the greatest reduction in tumor biomarkers and inflammation, as seen by decreased levels of Plasma malondialdehyde (MDA), tumor protein 53 (p53), Tumor necrosis factor-alpha (TNF- alpha), and fibronectin. Results concluded that these thorough characterizations, biological assessments, and antibacterial tests have confirmed the effective integration of SAE into CsNPs. Further, SAE loaded CsNPs could be a suitable option for various biomedical applications in tackling lung cancer and the inactivation of bacterial infection.