■人乳头瘤病毒(HPV)是全球最常见的性传播感染。宫颈阴道菌群在HPV感染中起重要作用,并与鳞状上皮内病变(SIL)的发展有关。宫颈癌的自然史涉及宫颈组织从正常状态的可逆变化,在鳞状上皮中没有检测到肿瘤的变化,细胞异常的不同状态最终导致宫颈癌。低等级SIL(LSIL),像另一个细胞学类别-不确定意义的非典型鳞状细胞(ASCUS),可能进展为高级别SIL(HSIL)和浸润性宫颈癌或可能恢复正常状态。
■在这项工作中,我们研究了165名HPV阳性和HPV阴性育龄期ASCUS妇女的宫颈管微生物组[HPV(+)n=29;HPV(-)n=11],LSIL[HPV(+)n=32;HPV(-)n=25],HSIL[HPV(+)n=46],对照组上皮内病变恶性(NILM)阴性[HPV(-)n=22]。
■HPV16是最流行的HPV类型。我们没有发现研究小组的多样性之间有任何差异,但有几个属[如普雷沃氏菌(p值=0.026),加德纳菌(p值=0.003),Fannyhessea(p值=0.024)]与NILM或LSIL相比,无论HPV如何,HSIL组中更常见。我们发现研究组中细菌属的发生率或比例存在统计学上的显着差异。我们还发现,增加乳酸杆菌的比例或患者的年龄导致更高的机会HSIL,而卷曲乳杆菌比例的增加导致LSIL的机会更高。中度菌群失调的患者通常具有三种类型的阴道微生物群落中的一种(CST,社区状态类型)与卷曲乳杆菌(CSTI)的患病率,加氏乳杆菌(CSTII),和乳酸杆菌(CSTIII);而严重的生态失调与CSTIV有关,涉及与细菌性阴道病和需氧性阴道炎相关的微生物属:加德纳菌,Fannyhessea,Dialister,Sneathia,缺氧球菌,Megasphaera,普雷沃氏菌,Finegoldia,Peptoniphilus,卟啉单胞菌,Parvimonas,和链球菌。
UNASSIGNED: Human papilloma virus (HPV) is the most common sexually transmitted infection worldwide. Cervicovaginal microbiota plays an important role in HPV infection and is associated with the development of squamous intraepithelial lesions (SIL). The natural history of cervical cancer involves reversible changes in the cervical tissue from a normal state, in which no neoplastic changes are detected in the squamous epithelium, to varying states of cellular abnormalities that ultimately lead to cervical cancer. Low-grade SIL (LSIL), like another cytological category - atypical squamous cells of undetermined significance (ASCUS), may progress to high-grade SIL (HSIL) and invasive cervical cancer or may regress to a normal state.
UNASSIGNED: In this work, we studied cervical canal microbiome in 165 HPV-positive and HPV-negative women of a reproductive age with ASCUS [HPV(+) n = 29; HPV(-) n = 11], LSIL [HPV(+) n = 32; HPV(-) n = 25], HSIL [HPV(+) n = 46], and the control group with negative for intraepithelial lesion malignancy (NILM) [HPV(-) n = 22].
UNASSIGNED: HPV16 is the most prevalent HPV type. We have not found any differences between diversity in studied groups, but several genus [like Prevotella (p-value = 0.026), Gardnerella (p-value = 0.003), Fannyhessea (p-value = 0.024)] more often occurred in HSIL group compared by NILM or LSIL regardless of HPV. We have found statistically significant difference in occurrence or proportion of bacterial genus in studied groups. We also identified that increasing of the ratio of Lactobacillus iners or age of patient lead to higher chance to HSIL, while increasing of the ratio of Lactobacillus crispatus lead to higher chance to LSIL. Patients with a moderate dysbiosis equally often had either of three types of vaginal microbial communities (CST, Community State Type) with the prevalence of Lactobacillus crispatus (CST I), Lactobacillus gasseri (CST II), and Lactobacillus iners (CST III); whereas severe dysbiosis is linked with CST IV involving the microorganisms genera associated with bacterial vaginosis and aerobic vaginitis: Gardnerella, Fannyhessea, Dialister, Sneathia, Anaerococcus, Megasphaera, Prevotella, Finegoldia, Peptoniphilus, Porphyromonas, Parvimonas, and Streptococcus.