目的:半月板祖细胞(MPCs)已被确定为半月板再生的有希望的候选者,在细胞水平上了解半月板损伤的修复机制至关重要。在这项研究中,我们使用差异粘附纤维连接蛋白(DAF)技术研究了从不同物种分离的MPCs的生物学特性。我们旨在表征不同物种中的MPC,并评估这些模型在未来半月板研究中的可行性。
方法:从大鼠新鲜消化的半月板中分离出MPCs,兔子,山羊,和使用DAF的人类细胞。生物学特性,包括扩散,菌落形成,多谱系分化,和迁移能力,在每个物种的MPCs及其相应的混合半月板细胞(MCs)种群中进行了比较。
结果:通过DAF技术在所有物种中成功分离出MPCs。大鼠MPCs看起来像鹅卵石,兔子MPCs更多是多边形的,山羊MPC具有纺锤形的形态,人的MPC看起来更像成纤维细胞。与MC相比,分离的MPCs表现出祖细胞特征,包括多谱系分化能力和MSC(间充质干细胞)标志物(CD166、CD90、CD44、Stro-1)表达。它们还高度表达纤连蛋白受体CD49e和CD49c。MPC还显示出更大的增殖能力和保留的集落形成能力。除了山羊MPC表现出比MC更大的迁移能力,在其他物种中,MPCs和MCs之间的迁移能力没有显着差异。
结论:我们的研究表明,DAF是从大鼠体内分离MPCs的有效方法,兔子,山羊,和人类。这些物种中的MPCs表现出相似的特征,包括更大的增殖能力和更好的软骨形成潜力。
OBJECTIVE: Meniscus progenitor
cells (MPCs) have been identified as promising candidates for meniscus regeneration, and it is crucial for us to understand meniscus injury repair mechanism at the cellular level. In this study, we investigate the biological properties of MPCs isolated from different species using the differential adhesion to fibronectin (DAF) technique. We aim to characterize MPCs in different species and evaluate the feasibility of these models for future meniscal investigation.
METHODS: MPCs were isolated from freshly digested meniscus from rat, rabbit, goat, and human
cells using DAF. Biological properties, including proliferation, colony-forming, multilineage differentiation, and migration abilities, were compared in MPCs and their corresponding mixed meniscus cell (MCs) population in each species.
RESULTS: MPCs were successfully isolated by the DAF technique in all species. Rat MPCs appeared cobblestone-like, rabbit MPCs were more polygonal, goat MPCs had a spindle-shaped morphology, human MPCs appear more fibroblast-like. Compared with MCs, isolated MPCs showed progenitor cell characteristics, including multilineage differentiation ability and MSC (mesenchymal stem
cells) markers (CD166, CD90, CD44, Stro-1) expression. They also highly expressed fibronectin receptors CD49e and CD49c. MPCs also showed greater proliferation capacity and retained colony-forming ability. Except for goat MPCs showed greater migration abilities than MCs, no significant differences were found in the migration ability between MPCs and MCs in other species.
CONCLUSIONS: Our study shows that DAF is an effective method for isolating MPCs from rat, rabbit, goat, and human. MPCs in these species demonstrated similar characteristics, including greater proliferation ability and better chondrogenic potential.