一些内生菌可以改善植物生长并抑制植物病害。然而,关于内生细菌促进小麦生长和抑制镰刀菌幼苗枯萎病病原体镰刀菌的潜力知之甚少。本研究旨在分离和鉴定内生细菌,并评估其对小麦镰刀菌枯萎病(FSB)的植物生长促进和病害抑制的功效。poae假单胞菌菌株CO在体外和温室条件下对F.graminearum菌株PH-1具有很强的抗真菌活性。P.poae菌株CO的无细胞上清液(CFSs)能够抑制菌丝体生长,形成菌落的数量,孢子萌发,胚芽管长度,FSB的霉菌毒素产生抑制率分别为87.00、62.25、51.33、69.29和71.08%,分别,CFS浓度最高。结果表明,P.poae表现出多种抗真菌特性,例如水解酶的生产,铁载体,和脂肽。此外,与未经处理的种子相比,用菌株处理的小麦植株表现出显著的生长速率,其中根和芽的长度增加了约33%,新鲜根的重量增加了,新鲜的枝条,干燥的根,干芽减少50%。此外,该菌株产生了高水平的吲哚-3-乙酸,磷酸盐溶解,和固氮。最后,该菌株表现出强烈的拮抗特性以及多种植物生长促进特性。因此,这一结果表明,该菌株可以用作合成化学品的替代品,可以作为保护小麦免受真菌感染的有效方法。
Some endophyte bacteria can improve plant growth and suppress plant diseases. However, little is known about the potential of endophytes bacteria to promote wheat growth and suppress the Fusarium seedling blight pathogen Fusarium graminearum. This study was conducted to isolate and identify endophytic bacteria and evaluate their efficacy for the plant growth promotion and disease suppression of Fusarium seedling blight (FSB) in wheat. The Pseudomonas poae strain CO showed strong antifungal activity in vitro and under greenhouse conditions against F. graminearum strain PH-1. The cell-free supernatants (CFSs) of P. poae strain CO were able to inhibit the mycelium growth, the number of colonies forming, spore germination, germ tube length, and the mycotoxin production of FSB with an inhibition rate of 87.00, 62.25, 51.33, 69.29, and 71.08%, respectively, with the highest concentration of CFSs. The results indicated that P. poae exhibited multifarious antifungal properties, such as the production of hydrolytic enzymes, siderophores, and lipopeptides. In addition, compared to untreated seeds, wheat plants treated with the strain showed significant growth rates, where root and shoot length increased by about 33% and the weight of fresh roots, fresh shoots, dry roots, and dry shoots by 50%. In addition, the strain produced high levels of indole-3-acetic acid, phosphate solubilization, and nitrogen fixation. Finally, the strain demonstrated strong antagonistic properties as well as a variety of plant growth-promoting properties. Thus, this result suggest that this strain could be used as an alternate to synthetic chemicals, which can serve as an effective method of protecting wheat from fungal infection.