最近,用于远程刺激机械力敏感和温度敏感蛋白以调节细胞内途径的物理工具为基础研究和临床应用开辟了新颖而令人兴奋的途径。在众多的物理刺激模式中,由于具有深度穿透和时空受控转导的优势,磁刺激对生物应用具有显着吸引力。在这里,物理化学参数(例如,形状,尺寸,组成),系统地总结了影响磁性纳米系统的磁性以及瞬时受体电位香草素1(TRPV1)和瞬时受体电位香草素4(TRPV4)通道的特征,这为以精确有效的方式对细胞命运进行磁性操纵提供了机会。此外,重点介绍了涉及基于磁性纳米系统的TRPV1和TRPV4通道激活的代表性监管应用,在细胞水平和动物模型中。此外,对这种磁刺激模式的进一步发展进行了评论,强调科学的局限性和可能的开发方向。本文分为:诊断工具>生物传感诊断工具>体内纳米诊断和成像。
Recently, physical tools for remotely stimulating mechanical force-sensitive and temperature-sensitive proteins to regulate intracellular pathways have opened up novel and exciting avenues for basic research and clinical applications. Among the numerous modes of physical stimulation, magnetic stimulation is significantly attractive for biological applications due to the advantages of depth penetration and spatial-temporally controlled transduction. Herein, the physicochemical parameters (e.g., shape, size, composition) that influence the magnetic properties of magnetic nanosystems as well as the characteristics of transient receptor potential vanilloid-1 (TRPV1) and transient receptor potential vanilloid-4 (TRPV4) channels are systematically summarized, which offer opportunities for magnetic manipulation of cell fate in a precise and effective manner. In addition, representative regulatory applications involving magnetic nanosystem-based TRPV1 and TRPV4 channel activation are highlighted, both at the cellular level and in animal models. Furthermore, perspectives on the further development of this magnetic stimulation mode are commented on, with emphasis on scientific limitations and possible directions for exploitation. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vivo Nanodiagnostics and Imaging.