两栖动物分类群包括三个具有不同形态特征的阶:Anura,Caudata,还有Apoda.它们的皮肤具有至关重要的作用:它作为构成身体的免疫器官,化学,免疫学,和微生物屏障对病原体的伤害和进行必要的生理过程。两栖动物已经开发出专门的功能来保护脆弱的皮肤屏障,包括皮肤表面下的腺体网络,可以产生抗菌和有毒物质,从而有助于防御病原体和捕食者。这项研究旨在表征Lithobatescatesbeianus皮肤中的Langerhans细胞(顺序:Anura;Shaw,1802),Amphiuma是指(订单:Caudata;花园,1821),和伤寒(顺序:Apoda;Fischer,1880)与以下抗体:Langerin/CD207(c型凝集素),主要组织相容性复合体(MHC)II,和Toll样受体(TLR)2(由不同类型的DC表达)。我们的结果表明,在这三个物种的表皮中,Langerhans细胞对LangerinCD/207呈阳性;此外,结缔组织中的一些抗原呈递细胞(APC)表达TLR2和MHCII。在所检查的三种两栖动物中,朗格汉斯细胞的分布非常相似,尽管他们的栖息地不同。对两栖动物免疫系统的更多了解可能有助于更好地了解脊椎动物的系统发育,并保护两栖动物免受人口下降的影响。此外,两栖动物和人类皮肤在免疫学特征方面的相似性可能在生物学和转化医学中都有用。
The amphibian taxon includes three orders that present different morphological characteristics: Anura,
Caudata, and Apoda. Their skin has a crucial role: it acts as an immune organ constituting a physical, chemical, immunological, and microbiological barrier to pathogen insult and conducts essential physiological processes. Amphibians have developed specialized features to protect the vulnerable skin barrier, including a glandular network beneath the skin surface that can produce antimicrobial and toxic substances, thus contributing to the defense against pathogens and predators. This study aims to characterize Langerhans cells in the skin of Lithobates catesbeianus (order: Anura; Shaw, 1802), Amphiuma means (order:
Caudata; Garden, 1821), and Typhlonectes natans (order: Apoda; Fischer, 1880) with the following antibodies: Langerin/CD207 (c-type lectin), Major Histocompatibility Complex (MHC)II, and Toll-like receptor (TLR)2 (expressed by different types of DCs). Our results showed Langerhans cells positive for Langerin CD/207 in the epidermis of the three species; moreover, some antigen-presenting cells (APCs) in the connective tissue expressed TLR2 and MHCII. The distribution of the Langerhans cells is very similar in the three amphibians examined, despite their different habitats. A greater knowledge of the amphibian immune system could be useful to better understand the phylogeny of vertebrates and to safeguard amphibians from population declines. Furthermore, the similarities between amphibians\' and human skin concerning immunological features may be useful in both biology and translational medicine.