胎盘是一个临时的母胎器官,和它的母体胎盘(carl)对胎儿的生长发育至关重要。胎盘的交换功能需要血管发育(血管生成)。然而,在妊娠早期,对山羊的血管生成知之甚少。这里,我们调查了血管分布,主要血管生成因子的mRNA表达,和ANGPT2在山羊肉梗中的甲基化水平。它表明CAD(毛细血管面积密度),CSD(毛细管表面密度),APC(每个毛细管的面积)逐渐增加,而CND(毛细管数密度)显示出微不足道的变化,可能是由于动物之间的差异。观察到增殖细胞的比例非常高(26%),并且从妊娠的第20天至第60天增加(p<0.002)约2倍。此外,主要血管生成因子的表达模式在妊娠早期发生改变。有趣的是,我们发现ANGPT2、TEK、FGF2和血管分布。随后,我们评估了ANGPT2的DNA甲基化,发现平均甲基化与CAD呈负相关.CpG位点的甲基化,CpG4/18、CpG9.10.11和CpG15在妊娠早期表现出显著变化。因此,我们的研究结果表明,ANGPT2的甲基化可能参与了妊娠早期动脉生成的调节。
The placenta is a temporary maternal−fetal organ, and its maternal placenta (
caruncle) is essential for fetal growth and development. The exchange function of the placenta requires vascular development (angiogenesis). However, the angiogenesis of the
caruncle is poorly understood in goats during the early stage of pregnancy. Here, we investigated the vascular distribution, mRNA expression of major angiogenic factors, and the methylation levels of ANGPT2 in the goat
caruncle. It showed that CAD (capillary area density), CSD (capillary surface density), and APC (area per capillary) increased gradually, while CND (capillary number density) showed an insignificant change, probably due to the variability between animals. The proportion of proliferating cells was observed to be very high (>26%) and increased (p < 0.002) approximately 2-fold from day 20 to 60 of pregnancy. Furthermore, the expression patterns of major angiogenic factors changed during the early stage of pregnancy. Interestingly, we discovered an absolute correlation between the mRNA for ANGPT2, TEK, FGF2, and vascular distribution. Subsequently, we evaluated the DNA methylation of ANGPT2, where we found that mean methylation was negatively correlated with CAD. The methylation at the CpG sites, such as CpG 4/18, CpG 9.10.11, and CpG 15, showed significant changes during the early stage of pregnancy. Thus, our findings suggest that the methylation of ANGPT2 may be involved in the regulation of
caruncle angiogenesis during the early stage of pregnancy.