未经评估:寻找光动力疗法的新制剂旨在使用显着减少的光敏剂剂量改善皮肤癌治疗的结果,从而避免副作用。将光敏剂掺入纳米组装体中是提高药物递送到靶细胞中的效率和特异性的通用方式。在这里,我们报告了玫瑰孟加拉装载到两亲性三嗪碳硅烷树突(树枝状)的囊泡状结构中,以及这种新型纳米系统的生物物理和体外表征。
UNASSIGNED:使用已建立的协议以及分析和光谱学技术,我们能够合成具有严格设计特性的树突。参与生物物理方法(流体动力学直径和ζ电位测量,光谱特性分析,透射电子显微镜)我们证实了我们的纳米系统的组装。一组体外技术用于测定ROS的产生,(ABDA和H2DCFDA探针),细胞活力(MTT测定)和细胞摄取(流式细胞术和共聚焦显微镜)。
未经授权:在树枝状分子内包裹玫瑰孟加拉可增强细胞摄取,细胞内ROS的产生和适当的,这种光敏剂的光毒性。
UNASSIGNED:三嗪-碳硅烷树枝状体显示出作为抗癌光动力疗法的药物载体的高容量。
UNASSIGNED: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-
carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem.
UNASSIGNED: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H2DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy).
UNASSIGNED: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer.
UNASSIGNED: Triazine-
carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.