背景:鲍曼不动杆菌是引起感染性疾病的重要医院病原菌之一。本研究旨在确定从设拉子患者中收集的碳青霉烯类耐药鲍曼不动杆菌的转座因子和分子分型与碳青霉烯类耐药基因的频率。伊朗。
方法:从两家医院的不同临床标本中共获得170株耐碳青霉烯类鲍曼不动杆菌。确定亚胺培南的最低抑制浓度(MIC)和OXA碳青霉烯酶的患病率,金属-β-内酰胺酶基因,插入序列(IS)元素,和转座子通过聚合酶链反应(PCR)方法进行评估。最后,通过肠杆菌重复基因间共有PCR方法对分离物进行分子分型.
结果:亚胺培南耐药鲍曼不动杆菌分离株的MIC范围为16至1,024µg/mL。在170种耐碳青霉烯类鲍曼不动杆菌分离物中,以blaOXA-24样(94,55.3%)为主,其次是blaOXA-23样(71,41.7%)。此外,鲍曼不动杆菌分离株携带蓝光(71,41.7%),blaGES(32,18.8%),blaSPM(4,2.3%),和blaKPC(1,0.6%)。此外,ISAba1(94.2%)和Tn2009(39.2%)是最常见的转座因子。此外,分离株的ISAba1(71,44.0%)和(161,94.7%)与blaOXA-23和blaOXA-51基因相关,分别。此外(3,1.7%),blaOXA-23的(1,0.6%)和(5,2.9%)分别与IS18,ISAba4和ISAba2相关。考虑到80.0%的截止,检测到簇和四个单例。
结论:根据结果,转座因子在碳青霉烯类抗病基因的产生和抗病中发挥了重要作用。结果还表明耐碳青霉烯类鲍曼不动杆菌是公共卫生问题。
BACKGROUND: Acinetobacter baumannii is one of the most important hospital pathogenic bacteria that cause infectious diseases. The present study aimed to determine the frequency of carbapenem resistance genes in association with transposable elements and molecular typing of carbapenem-resistant A. baumannii bacteria collected from patients in Shiraz, Iran.
METHODS: A total of 170 carbapenem-resistant A. baumannii isolates were obtained from different clinical specimens in two hospitals. The minimum inhibitory concentrations (MIC) of imipenem were determined and the prevalence of OXA Carbapenemases, Metallo-β-lactamases genes, insertion sequences (IS) elements, and transposons were evaluated by the polymerase chain reaction (PCR) method. Finally, molecular typing of the isolates was performed by the Enterobacterial Repetitive Intergenic Consensus-PCR method.
RESULTS: The MICs ranged from 16 to 1,024 µg/mL for imipenem-resistant A. baumannii isolates. Out of the 170 carbapenem resistant A. baumannii isolates, blaOXA-24-like (94, 55.3%) followed by blaOXA-23-like (71, 41.7%) were predominant. In addition, A. baumannii isolates carried blaVIM (71, 41.7%), blaGES (32, 18.8%), blaSPM (4, 2.3%), and blaKPC (1, 0.6%). Moreover, ISAba1 (94.2%) and Tn2009 (39.2%) were the most frequent transposable elements. Furthermore, (71, 44.0%) and (161, 94.7%) of the ISAba1 of the isolates were associated with blaOXA-23 and blaOXA-51 genes, respectively. Besides (3, 1.7%), (1, 0.6%) and (5, 2.9%) of blaOXA-23 were associated with IS18, ISAba4, and ISAba2, respectively. Considering an 80.0% cut off, clusters and four singletons were detected.
CONCLUSIONS: According to the results, transposable elements played an important role in the development of resistance genes and resistance to carbapenems. The results also indicated carbapenem-resistant A. baumannii bacteria as a public health concern.