背景:2,6-二取代的哌啶-3-醇是在番泻叶等物种中发现的一组重要的哌啶生物碱,其主要成分包括卡辛碱和针曲碱,具有相关药理活性的化合物。由于植物提取物的复杂性和不存在能够吸收紫外线(UV)辐射的发色团,因此对这些化合物的分析具有挑战性。
目的:本文提出了一种新的分析方法,该方法使用毛细管区带电泳(CZE)和间接UV检测技术分离和定量来自S花的乙醇提取物中存在的非UV吸收生物碱。
方法:优化的CZE方法采用含有60mM组氨酸(His)的背景电解质,15mMα-环糊精,20%乙腈(ACN),用乙酸(AcOH)调节pH至4.7。
结果:卡辛碱和针曲碱的检出限(LOD)值为10.2和13.9mgL-1,分别。对于这两种分析物,迁移次数和峰面积的精度数据优于2%的相对标准偏差(RSD)。为了评估所开发方法的适用性,制备并分析了来自S.spectabilis花的乙醇提取物。
结论:因此,该方法被证明是有效的,是对传统技术的补充,在定量植物提取物中存在的非紫外线吸收哌啶生物碱方面提供了一种具有成本效益的替代方法。
BACKGROUND: 2,6-Disubstituted piperidin-3-ols are an important group of piperidine alkaloids found in species such as Senna spectabilis, whose main constituents include cassine and spectaline, compounds with relevant pharmacological activity. The analysis of these compounds is challenging due to the complexity of plant extracts and the absence of chromophores capable of absorbing ultraviolet (UV) radiation.
OBJECTIVE: This paper presents a new analytical method to separate and quantify the non-UV-absorbing alkaloids present in ethanol extracts from S. spectabilis flowers using capillary zone electrophoresis (CZE) with indirect UV detection.
METHODS: The optimized CZE method employs a background electrolyte containing 60 mM histidine (His), 15 mM α-cyclodextrin, 20% acetonitrile (ACN), and pH-adjusted to 4.7 with acetic acid (AcOH).
RESULTS: The limit of detection (LOD) values was 10.2 and 13.9 mg L-1 for cassine and spectaline, respectively. For both analytes, the precision data were better than 2% of relative standard deviation (RSD) for migration times and peak areas. To evaluate the applicability of the developed method, ethanolic extracts from S. spectabilis flowers were prepared and analyzed.
CONCLUSIONS: Thereby, the method proved to be efficient and complementary to conventional techniques, offering a cost-effective alternative in the quantification of the non-UV-absorbing piperidine alkaloids present in plant extracts.