微生物已显示出生物修复制革废物污染土壤的潜力。在我们之前的研究中,观察到重金属抗性洋葱伯克霍尔德氏菌CS8增强了观赏植物的生长和植物修复能力。本研究工作的目的是评估洋葱CS8辅助金盏花植物对制革固体废物(TSW)污染土壤的植物修复能力。TSW处理显着降低了C的生长属性和光合色素。然而,补充对TSW改良土壤表现出实质性耐受性的洋葱芽孢杆菌CS8,增强的生长性状,类胡萝卜素,脯氨酸,和抗氧化酶水平在有毒和无毒的制度下。接种洋葱芽孢杆菌CS8可增强植物生长(芽长度13%,根长11%),生理属性(叶绿素a14%,叶绿素b17%),抗氧化酶活性(过氧化物酶24%,超氧化物歧化酶31%和过氧化氢酶19%),提高脯氨酸36%,苯酚32%,与TSW10处理的相关未接种植物相比,在TSW10胁迫下,黄酮类化合物14%,丙二醛(MDA)含量下降15%,过氧化氢(H2O2)水平下降12%。此外,B.洋葱CS8应用增强了土壤中的不稳定金属和随后的金属吸收,如Cr19%,Cd22%,Ni35%,铁18%,Cu21%,Pb34%,和锌30%,分别在受到TSW10胁迫的C.officinalis植物中,与未接种的类似处理相比。通过微生物接种,更高的植物胁迫耐受性和改善的植物修复潜力将有助于除了更新原生植被外,还有助于恢复农田。
在目前的研究中,观察到金盏花和耐金属洋葱伯克霍尔德氏菌CS8的组合不仅改善了植物的生长,而且还有助于植物提取制革固体废物污染土壤中存在的污染物。根据我们的信息,巴基斯坦尚未报道描述本地耐金属微生物和观赏植物的植物修复潜力的研究工作。
Microbes have shown potential for the bioremediation of tannery waste polluted soil. During our previous study, it was observed that heavy metal resistant Burkholderia cepacia CS8 augmented growth and phytoremediation capability of an ornamental plant. Objective of the present research work was to evaluate the capability of B. cepacia CS8 assisted Calendula officinalis plants for the phytoremediation of tannery solid waste (TSW) polluted soil. The TSW treatment significantly reduced growth attributes and photosynthetic pigments in C. officinalis. However, supplementation of B. cepacia CS8 which exhibited substantial tolerance to the TSW amended soil, augmented growth traits, carotenoid, proline, and antioxidant enzymes level in C. officinalis under toxic and nontoxic regimes. Inoculation of B. cepacia CS8 augmented plant growth (shoot length 13%, root length 11%), physiological attributes (chlorophyll a 14%, chlorophyll b 17%), antioxidant enzyme activities (peroxidase 24%, superoxide dismutase 31% and catalase 19%), improved proline 36%, phenol 32%, flavonoids 14% and declined malondialdehyde (MDA) content 15% and hydrogen peroxide (H2O2) level 12% in C. officinalis at TSW10 stress compared with relevant un-inoculated plants of TSW10 treatment. Moreover, B. cepacia CS8 application enhanced labile metals in soil and subsequent metal uptake, such as Cr 19%, Cd 22%, Ni 35%, Fe 18%, Cu 21%, Pb 34%, and Zn 30%, respectively in C. officinalis plants subjected to TSW10 stress than that of analogous un-inoculated treatment. Higher plant stress tolerance and improved phytoremediation potential through microbial inoculation will assist in the retrieval of agricultural land in addition to the renewal of native vegetation.
During the current study, it was observed that combination of Calendula officinalis and metal tolerant Burkholderia cepacia CS8 not only improved plant growth but also helped phyto-extraction of pollutants present in the tannery solid waste polluted soil. According to our information, research work describing the phytoremediation potential of native metal tolerant microbes and ornamental plants has not been reported in Pakistan.