■这项体内研究旨在检查当代硅酸钙水泥(CSC)在大鼠中含有不同的放射不透明剂的全身作用。
■填充有生物因子MTA(BIO)的聚乙烯管,NeoMTAPlus(NEO),MTA修复HP(REP),将生物牙本质(DENT)和空管(对照组)植入80只雄性SpraqueDawley大鼠的皮下组织中7天和30天(n=8)。经过7天和30天,肝脏和肾脏组织样本进行组织病理学分析。收集血样以评估大鼠肝和肾功能的变化。使用Wilcoxon和事后DunnBonferroni测试在第7天和第30天之间进行比较,以评估组织病理学数据。使用配对样本t检验比较第7天和第30天的实验室值,使用ANOVA分析和事后Tukey检验来比较组间的值(p<0.05)。
■第7天,代表,BIO和NEO组在肾脏组织中具有统计学相似,并且发现这些组的炎症程度明显高于对照组和DENT组。在第30天,发现REP和NEO组在肾组织中的炎症程度明显高于对照组,BIO和DENT组。尽管在第7天和第30天肝脏的炎症是中度和轻度的,两组间无统计学差异.各组肾脏和肝脏血管充血评价为轻度和中度,组间差异无统计学意义。虽然在第7天AST中各组之间没有统计学上的显着差异,ALT和尿素值,当比较肌酐值时,发现DENT组和NEO组在统计学上相似,并且显著低于对照组.在第30天,各组之间的ALT值在统计学上相似。发现BIO组的AST值显著高于DENT组。虽然生物,DENT,NEO和对照组的尿素值在统计学上相似,发现REP组显著高于其他组.REP组肌酐值明显高于对照组(p<0.05)。
■不同放射遮光剂的CSC对全身肾脏和肝脏的组织学检查具有相似且可接受的效果,和血清ALT,AST,尿素,肌酐水平。
This in vivo study aimed to examine the systemic effects of contemporary calcium silicate cements (CSC) contain different radiopacifiers in rats.
Polyethylene tubes filled with BIOfactor MTA (BIO), Neo MTA Plus (NEO), MTA Repair HP (REP), Biodentine (DENT) and empty tubes (control group) were implanted into the subcutaneous tissues of 80 male Spraque Dawley rats for 7 and 30 days (n = 8). After 7 and 30 day, samples of liver and kidney tissues were submitted to histopathological analysis. Blood samples were collected to evaluate changes in hepatic and renal functions of rats. Wilcoxon and post hoc Dunn Bonferroni tests were used to compare between the 7th and 30th days in order to evaluate the histopathological data. Paired-sample t-test was used to compare laboratory values between the 7th and 30th days, ANOVA analysis and a post hoc Tukey test were used to compare values between groups (p < 0.05).
On the 7th day, REP, BIO and NEO groups were statistically similar in kidney tissue and the degree of inflammation was found to be significantly higher in these groups compared to the control and DENT groups. On the 30th day, the degree of inflammation of the REP and NEO groups in the kidney tissue was found to be significantly higher than the control, BIO and DENT groups. Although the inflammation in the liver was moderate and mild on the 7th and 30th days, no statistically significant difference was observed between the groups. Vascular congestion was evaluated as mild and moderate in kidney and liver in all groups, and no statistically significant difference was observed between the groups. While there was no statistically significant difference between the groups in the 7th day AST, ALT and urea values, when the creatinine values were compared, the DENT and NEO groups were found to be statistically similar and significantly lower than the control group. On the 30th day, ALT values were statistically similar between the groups. The AST values of the BIO group were found to be significantly higher than the DENT group. While BIO, DENT, NEO and control groups had statistically similar urea values, the REP group was found to be significantly higher than the other groups. The creatinine value of the REP group was significantly higher than the groups other than the control group (p < 0.05).
CSCs with different radiopacifiers had similar and acceptable effects on the histological examination of the kidneys and liver systemically, and serum ALT, AST, urea, creatinine levels.