在这项研究中,验证了高压辅助液体萃取从牛皮废料中制备氧化明胶的可行性。不同的加工条件,包括高压时间(15至25分钟),压力(250至350MPa),和液固比(1:3至1:5),通过响应面法进行了优化。在最佳制造条件下,当高压处理(HPP)时间为22分钟时,压力为289兆帕,液固比为1:4时,提取率最高(36%),凝胶强度最高(224g)。基于DSC,XRD,FTIR,SEM,胶凝和熔化温度,HPP导致糊化胶原蛋白的结构修饰,在凝胶化过程中增强了凝胶结构的重排,使其具有更好的胶凝性能。此外,与商业样本相比,它们在制备的氧化物明胶的弛豫时间和峰面积上没有显着差异。这些发现为HPP在制备氧化物明胶过程中的实用性提供了新的见解,可显著缩短加工时间,应用于工业生产。实际应用:与传统加工相比,高压辅助提取工艺可以显着减少处理时间,同时产生具有相似物理化学和功能特性的牛皮凝胶。同时,高压处理(HPP)导致牛皮和糊化胶原蛋白的结构破坏,这增强了明胶在凝胶化过程中结构的重排,使其具有更好的胶凝性能。重要的是,高压辅助提取可以方便地使用低成本的原料,提高明胶的制备效率,在大规模高效的工业生产和氧化明胶的质量控制方面显示出巨大的潜力。
In this study, the feasibility of preparing oxhide gelatin from cowhide scrap by high pressure assisted-liquid extraction was verified. Different processing conditions, including high pressure time (15 to 25 min), pressure (250 to 350 MPa), and liquid-to-solid ratio (1:3 to 1:5), were optimized through response surface methodology. Under the optimum manufacturing conditions, when the high-pressure processing (HPP) time was 22 min, the pressure was 289 MPa, and the liquid-to-solid ratio was 1:4, the highest extraction yield (36%) and gel strength (224 g) were achieved. Based on DSC, XRD, FTIR, SEM, gelling and melting temperatures, HPP led to the structural modification of the gelatinized collagen, which enhanced the rearrangement of the gel structure during the gelation process and made it have better gelling properties. In addition, compared with the commercial sample, they do not differ significantly in the relaxation time and peak area of prepared oxhide gelatin. These findings provide new insights into the practicability of HPP during the preparation of oxhide gelatin, which can noticeably reduce the processing time and be applied to industrial production. PRACTICAL APPLICATION: Compared with traditional processing, a high pressure-assisted extraction process can noticeably reduce the processing time while producing cowhide gelation with similar physicochemical and functional properties. Meanwhile, high pressure processing (HPP) led to the structural destruction of the cowhide and gelatinized collagen, which enhanced the rearrangement of the gelatin structure during the gelation process and made it have better gelling properties. Importantly, high pressure-assisted extraction can facilitate the use of a low-cost raw material and improve the preparation efficiency of oxhide gelatin, which shows great potential in large-scale and efficient industrial production and the quality control of oxhide gelatin.