■金黄色葡萄球菌(S.金黄色葡萄球菌)是烧伤伤口感染的最常见病原体,这往往导致高发病率和死亡率。然而,福建省对烧伤创面感染金黄色葡萄球菌的分子流行病学和抗菌药物敏感性的认识不足,中国。
■在2016年至2021年之间,从福建的烧伤伤口感染中收集了90株金黄色葡萄球菌,中国,其中耐甲氧西林(MRSA)菌株59株,耐甲氧西林(MSSA)菌株31株。这些被研究的分子特征,毒力基因,生物膜,和抗菌敏感性。采用多位点序列分型(MLST)对所有分离株进行基因分型,spa打字,agr打字,和SCCmec打字。进行常规PCR以检测毒力基因。通过组织培养板测定(TCP)评估生物膜形成能力。使用稀释法评估分离物的抗菌敏感性。
■总共,根据多位点序列分型(MLST)和spa分型鉴定出37种序列类型(ST)和34种葡萄球菌蛋白A(spa)类型(包括一种名为spa-t20720的新类型)。分别。CC8-ST239-t030-agrI-SCCmecIII(57.6%,34/59)和CC7-ST7-t091-agrI(16.1%,5/31)代表了MRSA和MSSA分离株的主要克隆,分别。抗生素敏感性测试发现,ST239和非ST239分离株之间的耐药率存在显着差异(p<0.05)。检测到12个毒力基因,其中最常见的是icaA和icaD(均为100%),其次是icaB和icaC(均为96.7%),icaR(95.6%),lukED(81.1%),卢卡(62.2%),pvl(50%),hlgBC(26.7%),和eta(4.4%)。此外,LukAB,hlgBC,agrI,和agrIII与烧伤严重程度显着相关(p<0.05)。MRSA分离株的可能性较小,与MSSA分离株相比,携带pvl,LukAB,和hlgBC(p<0.05)。一种新的温泉类型,t20720,被识别为包含pvl,luked,LukAB,hlgBC,icaA,icaB,icaC,icaD,和icaR基因并具有较强的生物膜形成能力。
■CC8-ST239-t030-agrI-SCCmecIII和CC7-ST-7-t091-agrI是福建烧伤创面感染的MRSA和MSSA分离株的普遍分子特征,中国,分别。新发现的spa-t20720分离株,携带广泛的毒力基因,具有很强的生物膜形成能力,需要特别的临床关注。
UNASSIGNED: Staphylococcus aureus (S. aureus) is the most common causative agent of burn wound infection, that often leads to high morbidity and mortality. However, there is not enough knowledge about the molecular epidemiology and antimicrobial susceptibility of S. aureus isolates from burn wound infections in Fujian, China.
UNASSIGNED: Between 2016 and 2021, 90 S. aureus isolates were collected from burn wound infections in Fujian, China, including 59 methicillin-resistant (MRSA) strains and 31 methicillin-susceptible (MSSA) strains. These were investigated for molecular characteristics, virulence genes, biofilms, and antimicrobial susceptibility. All the isolates were genotyped by multilocus sequence typing (MLST), spa typing, agr typing, and SCCmec typing. Conventional PCR was performed for the detection of virulence genes. Biofilm formation capacity was assessed by tissue culture plate assay (TCP). The antimicrobial susceptibility of the isolates was evaluated using the dilution method.
UNASSIGNED: In total, 37 sequence types (ST) and 34 Staphylococcal protein A (spa) types (including a new type named spa-t20720) were identified based on multilocus sequence typing (MLST) and spa typing, respectively. CC8-ST239-t030-agrI-SCCmecIII (57.6%,34/59) and CC7-ST7-t091-agrI (16.1%, 5/31) represented the main clone of MRSA and MSSA isolates, respectively. Antibiotic susceptibility testing identified a significant difference in resistance rates between ST239 and non-ST239 isolates (p < 0.05). Twelve virulence genes were detected, of which the most common were icaA and icaD (both 100%), followed by icaB and icaC (both 96.7%), icaR (95.6%), lukED (81.1%), lukAB (62.2%), pvl (50%), hlgBC (26.7%), and eta (4.4%). Moreover, lukAB, hlgBC, agrI, and agrIII were significantly correlated with burn severity (p < 0.05). MRSA isolates were less likely, compared with MSSA isolates, to carry pvl, lukAB, and hlgBC (p < 0.05). A new spa type, t20720, was identified that contains pvl, lukED, lukAB, hlgBC, icaA, icaB, icaC, icaD, and icaR genes and has strong biofilm formation ability.
UNASSIGNED: CC8-ST239-t030-agrI-SCCmecIII and CC7-ST-7-t091-agrI were the prevalent molecular signatures of MRSA and MSSA isolates from burn wound infections in Fujian, China, respectively. The newly identified spa-t20720 isolate, which carries a wide range of virulence genes and has strong biofilm formation ability, requires special clinical attention.