■亚麻籽油(FO)和维生素E(VE)都对精子具有抗氧化作用。本研究调查了膳食补充FO和/或VE对精液质量的影响。
■选择16头可育的西门塔尔公牛,随机分为4组(n=4):对照组(对照饮食),FO组(含24g/kgFO的对照饮食),VE组(含150mg/kgVE的对照饮食)和FOVE组(含150mg/kgVE和24g/kgFO的对照饮食),审判持续了10周.
■结果表明,独立地添加FO可以增加精子运动参数,过氧化氢酶(CAT)的水平,谷胱甘肽过氧化物酶(GSH-Px),睾酮(T)和雌二醇(E2),降低精浆氧化应激水平(P<0.05)。独立补充VE可以增加运动,运动性参数,CAT和超氧化物歧化酶(SOD)水平,降低精浆氧化应激水平(P<0.05)。FO×VE对运动性和活性氧(ROS)有交互作用,而GSH-Px和ROS受到周×VE双向相互作用的影响,T和E2水平也受饲粮FO×周交互作用的影响(P<0.05)。FO的三重相互作用效应,VE和周对丙二醛(MDA)有统计学意义(P<0.05)。与对照组相比,来自FOVE组的精子有明显更高的体外受精(IVF)率,随后的胚胎在八细胞阶段发育能力增强,ROS水平降低,然后增加三磷酸腺苷(ATP)含量和CAT的基因表达水平,CDX2,Nanog,胚泡期SOD(P<0.05)。代谢组学和转录组学结果表明,膳食补充FO和VE增加了代谢物乌头酸的表达,以及ABAT和AHDHA基因的表达。
■使用计算机内分析,可以得出结论,日粮FO和VE改善精液质量和胚胎发育的作用可能与通过参与丙酸代谢途径的ABAT和AHDHA基因增加乌头酸有关。
Flaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.
16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.
The results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.
With in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.