小麦是全世界种植的重要食品。然而,这种作物面临着来自真菌病原体的持续威胁,叶锈病(LR)和条锈病(YR)。为了继续养活不断增长的人口,必须通过提高栽培种质的遗传多样性来有效对抗小麦的这些主要破坏者。在这项研究中,具有六倍体背景的基因渗入系(ILsp3603),具有对Pt致病型77-5(121R63-1)的抗性,77-9(121R60-1)和Pst病理型46S119(46E159),110S119(110E159),238S119(238E159)是从供体小麦野生祖细胞开发的,Aegilopsspeltoidesaccpau3603。为了了解抗性的遗传基础并绘制这些基因(命名为Lrsp3603和Yrsp3603),在F6和F7作图种群中进行了遗传研究,通过将ILsp3603与LR和YR易感品种WL711杂交而开发,揭示了这些性状中每个性状的单基因(单基因)遗传模式。批量分离分析与35KAxiomSNP阵列基因分型相结合,将两个基因定位为6B染色体短臂上的独立实体。遗传连锁图谱,包括五个标记,1个SNP,1个PLUG和三个基于基因的SSRs,遗传距离为12.65cM。Lrsp3603的侧翼是标记物Tag-SSR14(位于2.42cM附近)和SNPAX-94542331(在3.28cM),而Yrsp3603的一端最靠近AX-94542331,距离6.62cM。Lrsp3603靶区域(〜1Mbp)的功能注释揭示了与抗病机制相关的10个基因ID,包括三个编码典型的R基因结构域。
Wheat is an essential food commodity cultivated throughout the world. However, this crop faces continuous threats from fungal pathogens, leaf rust (LR) and stripe rust (YR). To continue feeding the growing population, these major destructors of wheat must be effectively countered by enhancing the genetic diversity of cultivated germplasm. In this study, an introgression line with hexaploid background (ILsp3603) carrying resistance against Pt pathotypes 77-5 (121R63-1), 77-9 (121R60-1) and Pst pathotypes 46S119 (46E159), 110S119 (110E159), 238S119 (238E159) was developed from donor wheat wild progenitor, Aegilops speltoides acc pau 3603. To understand the genetic basis of resistance and map these genes (named Lrsp3603 and Yrsp3603), inheritance studies were carried out in F6 and F7 mapping population, developed by crossing ILsp3603 with LR and YR susceptible cultivar WL711, which revealed a monogenic (single gene) inheritance pattern for each of these traits. Bulk segregant analysis combined with 35 K Axiom SNP array genotyping mapped both genes as separate entities on the short arm of chromosome 6B. A genetic linkage map, comprising five markers, 1 SNP, 1 PLUG and three gene based SSRs, covered a genetic distance of 12.65 cM. Lrsp3603 was flanked by markers Tag-SSR14 (located proximally at 2.42 cM) and SNP AX-94542331 (at 3.28 cM) while Yrsp3603 was mapped at one end closest to AX-94542331 at 6.62 cM distance. Functional annotation of Lrsp3603 target region (∼ 1 Mbp) revealed 10 gene IDs associated with disease resistance mechanisms including three encoding typical R gene domains.