背景:烟叶科物种在纳米技术学科的研究人员中引起了人们的兴趣,因为它们具有多种生物活性,如抗炎,抗氧化剂,抗微生物,和抗肿瘤。在本研究中,研究了Polyclatiacrinita提取物和绿色合成的Polyclatiacrinita硒纳米颗粒(PCSeNPs)对乳腺癌细胞系(MDA-MB-231)和实体埃利希癌(SEC)的抗癌特性。方法:确定了Polycladiacrinita的气相色谱-质谱检查,并采用了各种分析方法,如SEM,TEM,EDX,和XRD,用于表征生物合成的PCSeNP。体外,使用针对MDA-MB-231的活力测定法评估了游离Polyclatiacrinita和PCSeNP的抗癌活性,并通过流式细胞术确定了细胞周期分析。此外,为了研究体内抗肿瘤作用背后的可能机制,将携带SEC的小鼠随机分为6组(n=6).第1组:肿瘤对照组,第2组:免费SeNPs,第3组:25毫克/千克丁香,组4:50mg/kgPolyclatiacrinita,组5:25mg/kgPCSeNP,组6:50mg/kgPCSeNP。结果:Polycladiacrinita提取物的气相色谱-质谱检查暴露了许多生物活性化合物的存在,如4-十八烯酸甲酯,十四烷酸,和正十六碳烯酸。这些化合物与其他化合物一起发现,可能会协同工作,以鼓励抗肿瘤活动的发展。Polyclatiacrinita提取物和PCSeNP被证明可以抑制癌细胞活力和早期细胞周期停滞。浓度为50mg/kg的PCSeNP显示抑制COX-2,NF-κB,VEGF,ki-67、Notch1和Bcl-2蛋白水平。否则,显示半胱天冬酶3,BAX的扩增,和P53蛋白水平。此外,caspase3,caspase9,Notch1,cyclinD1,NF-κB,IL-6和VEGF与PCSeNP相比于相似剂量的游离提取物显著更有效。结论:PCSeNPs通过增强细胞凋亡和减轻炎症来介导它们有希望的抗癌作用。表现为促进总生存率和肿瘤体积减小。
Background: Phaeophyceae species are enticing interest among researchers working in the nanotechnology discipline, because of their diverse biological activities such as anti-inflammatory, antioxidant, anti-microbial, and anti-tumor. In the present study, the anti-cancer properties of Polycladia crinita extract and green synthesized Polycladia crinita selenium nanoparticles (PCSeNPs) against breast cancer cell line (MDA-MB-231) and solid Ehrlich carcinoma (SEC) were investigated. Methods: Gas chromatography-mass spectroscopy examinations of Polycladia crinita were determined and various analytical procedures, such as SEM, TEM, EDX, and XRD, were employed to characterize the biosynthesized PCSeNPs. In vitro, the anticancer activity of free Polycladia crinita and PCSeNPs was evaluated using the viability assay against MDA-MB-231, and also cell cycle analysis by flow cytometry was determined. Furthermore, to study the possible mechanisms behind the in vivo anti-tumor action, mice bearing SEC were randomly allocated into six equal groups (n = 6). Group 1: Tumor control group, group 2: free SeNPs, group 3: 25 mg/kg Polycladia crinita, group 4: 50 mg/kg Polycladia crinita, group 5: 25 mg/kg PCSeNPs, group 6: 50 mg/kg PCSeNPs. Results: Gas chromatography-mass spectroscopy examinations of Polycladia crinita extract exposed the presence of many bioactive compounds, such as 4-Octadecenoic acid-methyl ester, Tetradecanoic acid, and n-Hexadecenoic acid. These compounds together with other compounds found, might work in concert to encourage the development of anti-tumor activities. Polycladia crinita extract and PCSeNPs were shown to inhibit cancer cell viability and early cell cycle arrest. Concentrations of 50 mg/kg of PCSeNPs showed suppression of COX-2, NF-кB, VEGF, ki-67, Notch 1, and Bcl-2 protein levels. Otherwise, showed amplification of the caspase 3, BAX, and P53 protein levels. Moreover, gene expression of caspase 3, caspase 9, Notch 1, cyclin D1, NF-кB, IL-6, and VEGF was significantly more effective with PCSeNPs than similar doses of free extract. Conclusion: The PCSeNPs mediated their promising anti-cancerous action by enhancing apoptosis and mitigating inflammation, which manifested in promoting the total survival rate and the tumor volume decrease.