目的:人乳(HM)的脂肪酸供应有助于健康结果。取样新鲜人乳以分析其脂肪酸含量是具有挑战性的,因为其不断变化的性质。此外,从哺乳期母亲那里获取样本是一项挑战。因此,促进HM收集和分析是一个优点。
方法:我们进行了一项研究,以验证一种获取HM样品进行脂肪酸分析的新方法,使用生物流体样品收集预处理的片材来吸附牛奶滴(Whatman903BHT预处理的生物流体收集片材)作为收集挤出的牛奶的替代方法。研究人群包括哺乳期母亲,分娩后24至96小时登记。
结果:使用两种不同的方法分析了总共124份母乳样品。游离乳分析的结果与吸附乳样品的分析相当。脂肪酸家族饱和脂肪酸(SFA),单不饱和脂肪酸(MUFA),多不饱和脂肪酸(PUFA),omega-3和omega-6的r2值分别为0.93、0.91、0.91、0.86和0.90。Bland-Altman地块显示,SFA的新鲜牛奶样品和吸附牛奶样品之间具有很高的一致性,MUFA,PUFA,omega-3和omega-6的平均偏差<2%,95%的一致性限制在-5%和+5%之内。
结论:结果表明,新鲜和吸附牛奶样品的脂肪酸组成没有显着差异,这表明新方法在收集代表性样品进行分析方面同样有效。
OBJECTIVE: The fatty acid supply of human milk (HM) contributes to health outcomes. Sampling fresh human milk to analyze its fatty acid content is challenging because of its ever-changing nature. Also, obtaining samples from lactating mothers is challenging. Facilitating HM collection and analysis is therefore an advantage.
METHODS: We have conducted a study to validate a new method for obtaining HM samples for fatty acid analysis, using biological fluid sample collection pretreated sheets to adsorb drops of milk (Whatman 903 BHT-pretreated biological fluid collection sheet) as an alternative approach to collecting expressed milk. The study population included lactating mothers, enrolled between 24 and 96 h after delivery.
RESULTS: A total of 124
breastmilk samples were analyzed using the two distinct approaches. The results of the free milk analysis were comparable to the analysis of adsorbed milk samples. The fatty acid families saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), omega-3, and omega-6 had r2 values of 0.93, 0.91, 0.91, 0.86, and 0.90, respectively. Bland-Altman plots showed a high agreement between fresh and adsorbed milk samples for SFA, MUFA, PUFA, omega-3, and omega-6 with a mean bias <2% and 95% limits of agreement within -5% and +5%.
CONCLUSIONS: The results show no significant differences in fatty acid composition between fresh and adsorbed milk samples, suggesting the new method is equally effective in collecting representative samples for analysis.