Finalyse,T4噬菌体,是一种收获前干预措施,利用噬菌体的组合,通过破坏进入商业屠宰场的收获准备牛的皮上的细菌来减少传入的大肠杆菌O157:H7的流行。这项研究的目的是评估Finalyse的疗效,作为收获前的干预,减少病原体,特别是大肠杆菌O157:H7,在牛皮和巢穴环境中,以整体减少传入的病原体负荷。超过5个采样事件,使用25mL预水合缓冲蛋白胨水(BPW)拭子采集总共300个复合生皮样品,在皮革清洗干预之前和之后收集,一开始,中间,和生产日结束(n=10拭子/采样点/时间点)。在生产日结束时,还同时采集了171个靴子拭子样品,方法是以预定的“Z”模式从笔的前面走到后面,以监测来自3个不同位置的笔地板环境。使用BAX®系统实时PCR测定分析病原体的流行率。在噬菌体施用后,在生皮上没有观察到沙门氏菌和/或任何产生志贺毒素的大肠杆菌(STEC)的显著减少(p>0.05)。在整个研究中,大肠杆菌O157:H7和O111的患病率非常低;因此,未进行进一步分析.然而,靴拭子监测显示在围栏地板环境中大肠杆菌O157:H7、O26和O45显著减少(p<0.05)。在商业牛肉加工设施的莱尔区使用Finalyse作为收获前干预措施时,这种噬菌体未能减少肉牛生皮上的大肠杆菌O157:H7,由于患病率较低;然而,一些STEC在巢穴环境中减少,应用噬菌体的地方。总的来说,关于Finalyse的有效性及其在肉牛生皮上减少大肠杆菌O157:H7的能力,没有形成绝对的结论,因为皮革的患病率很低。
Finalyse, a T4 bacteriophage, is a pre-harvest intervention that utilizes a combination of bacteriophages to reduce incoming Escherichia coli O157:H7 prevalence by destroying the bacteria on the hides of harvest-ready cattle entering commercial abattoirs. The objective of this study was to evaluate the efficacy of Finalyse, as a pre-harvest intervention, on the reduction in pathogens, specifically E. coli O157:H7, on the cattle hides and lairage environment to overall reduce incoming pathogen loads. Over 5 sampling events, a total of 300 composite hide samples were taken using 25 mL pre-hydrated Buffered Peptone Water (BPW) swabs, collected before and after the hide wash intervention, throughout the beginning, middle, and end of the production day (n = 10 swabs/sampling point/timepoint). A total of 171 boot swab samples were also simultaneously taken at the end of the production day by walking from the front to the back of the pen in a pre-determined \'Z\' pattern to monitor the pen floor environment from 3 different locations in the lairage area. The prevalence of pathogens was analyzed using the BAX® System Real-Time PCR Assay. There were no significant reductions observed for Salmonella and/or any Shiga toxin-producing E. coli (STEC) on the hides after the bacteriophage application (p > 0.05). Escherichia coli O157:H7 and O111 hide prevalence was very low throughout the study; therefore, no further analysis was conducted. However, boot swab monitoring showed a significant reduction in E. coli O157:H7, O26, and O45 in the pen floor environment (p < 0.05). While using Finalyse as a pre-harvest intervention in the lairage areas of commercial beef processing facilities, this bacteriophage failed to reduce E. coli O157:H7 on the hides of beef cattle, as prevalence was low; however, some STECs were reduced in the lairage environment, where the bacteriophage was applied. Overall, an absolute conclusion was not formed on the effectiveness of Finalyse and its ability to reduce E. coli O157:H7 on the hides of beef cattle, as prevalence on the hides was low.