在这项研究中,采用宏基因组测序技术分析了不同程度的茶叶和健康茶叶中内生真菌ITSrDNA基因的ITS1区序列和内生细菌的16S序列。随后,对茶叶内生微生物多样性和群落结构进行了比较分析。这项研究的结果表明,随着疾病的发展,优势内生真菌属从子囊菌向担子菌转移。此外,外植体和塔拉霉素之间呈负相关,具有Talaromyce作为该疾病的拮抗剂的潜力。同时,我们的发现揭示了变形杆菌,Firmicutes,放线菌是茶叶中最丰富的三种细菌门。随着疾病的发展,放线菌的相对丰度增加,而Variovorax,鞘氨醇单胞菌,和假单胞菌在后期发现具有更高的丰度。多样性分析结果表明,病组茶叶内生微生物多样性和群落结构均低于健康对照组。总的来说,叶枯病改变了茶叶内生微生物的群落结构,导致少数物种的丰度很高。该研究为研究茶树水疱病的致病机理和建立控制茶树病害的理论依据奠定了基础。
In this study, metagenomic sequencing technology was employed to analyze the ITS1 region sequence of the ITS rDNA gene of endophytic fungi and 16S sequence of endophytic bacteria in tea leaves with varying degrees of infection by tea blister blight disease as well as healthy tea leaves. Subsequently, a comparative analysis was conducted on the endophytic microbial diversity and the community structure in tea leaves. The findings of this investigation reveal a shift in the dominant endophytic fungal genera from Ascomycota to Basidiomycota as the disease progressed. Furthermore, a negative correlation was observed between Exobasidium and Talaromyce, with Talaromyce exhibiting potential as an antagonist against the disease. Meanwhile, our findings reveal that Proteobacteria, Firmicutes, and Actinobacteria were the three most abundant bacteria phyla in tea leaves. As the disease progressed, there was an increase in the relative abundance of Actinobacteria, while Variovorax, Sphingomonas, and Pseudomonas were found to have higher abundance in later stages. The diversity analysis results indicated that the endophytic microbial diversity and the community structure in tea leaves in the diseased group were lower than those in the healthy control group. In general, blister blight disease altered the community structure of endophytic microorganisms in tea leaves, resulting in a few species with high abundance. The study lays a foundation for investigating the pathogenic mechanism of tea blister disease and establishing a theoretical basis for controlling diseases in tea trees.